| 马铃薯茎尖组织培养方法优化研究 |
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| 引用本文： | 王桂梅,杨林栋.马铃薯茎尖组织培养方法优化研究[J].安徽农学通报,2012,18(9):48-49. |
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| 作者姓名： | 王桂梅 杨林栋 |
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| 作者单位： | 山西省农科院高寒区作物研究所,山西大同,037008;山西省农科院高寒区作物研究所,山西大同,037008 |
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| 摘 要： | 提高马铃薯茎尖组织培养的成苗率和脱毒率。以克新1号为材料,研究了不同茎尖大小、不同浓度和配比的激素对马铃薯茎尖诱导的效果。结果表明:茎尖越小,脱毒率越高,但成活率和成苗率越低,以叶原基数为2的茎尖脱毒效果最好。通过对6种培养基的对比,筛选出MS+6-BA 0.5 mg/L+NAA 0.1 mg/L+GA 0.1 mg/L为茎尖诱导分化成苗的最优培养基。
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| 关 键 词： | 马铃薯 茎尖组织 茎尖培养 |
Study on Optimizing Method of Potato Stem tip Tissue Culture |
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| Affiliation: | Wang Guimei et al. (High latitude and Cold Weather Crops Institute, Shanxi Academy of Agricultural Sciences, Datong 037008, China) |
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| Abstract: | To improve the potato stem tip tissue culture seedling rate and detoxication rate. Using the new 1 as material, study the effect of different size of shoot tip, concentrations and proportions of hormone on potato stem tip induction. The re- suits show that the smaller stem apex, virus -free rate is higher, but the survival rate and seedling rate is lower, with Ye Yuan base 2 stem apex detoxification effect best. Through the 6 medium contrast, screening out MS ＋ 6 - BA 0. 5 mg/L ＋ NAA 0. 1 mg/L ＋ GA 0. 1 mg/L stem tip induction seedling differentiation of optimal culture medium. |
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| Keywords: | Potato Stem tip tissue Meristem tip |
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