| 过氧亚硝酸诱导生成硫中心自由基的荧光表征 |
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| 引用本文： | 党亚敏,郭祥群. 过氧亚硝酸诱导生成硫中心自由基的荧光表征[J]. 高等学校化学学报, 2006, 27(3): 424-427 |
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| 作者姓名： | 党亚敏 郭祥群 |
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| 作者单位： | 教育部现代分析科学重点实验室,化学生物学福建省重点实验室,厦门大学化学系,厦门 361005 |
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| 摘 要： | 将4-羟基-2,2,6,6-四甲基哌啶氮氧自由基用于标记9-羧基-吖啶,得到自旋标记荧光探针4-(9-吖啶酯)-2,2,6,6-四甲基哌啶氮氧自由基. 以谷胱甘肽作为蛋白质肽模型,研究了活性氧过氧亚硝酸诱导其损伤产生的硫中心自由基的荧光表征. 自旋标记荧光探针为弱荧光物质,当与硫中心自由基作用后,导致其荧光增强,从而可对硫中心自由基进行表征.
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| 关 键 词： | 自旋标记 荧光探针 硫自由基 过氧亚硝酸, |
| 文章编号： | 0251-0790（2006）03-0424-04 |
| 收稿时间： | 2005-06-27 |
| 修稿时间： | 2005-06-27 |
Fluorescent Characterization of Peroxynitrite-induced Thiyl Radicals |
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| DANG Ya-Min,GUO Xiang-Qun. Fluorescent Characterization of Peroxynitrite-induced Thiyl Radicals[J]. Chemical Research In Chinese Universities, 2006, 27(3): 424-427 |
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| Authors: | DANG Ya-Min GUO Xiang-Qun |
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| Affiliation: | Department of Chemistry,the Key Laboratory of Analytical Science of MOE,; Xiamen University,Xiamen 361005,China |
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| Abstract: | A spin-labeling fluorophore(Ac-Tempo·),formed by combining with an acridine with a paramagnetic nitroxide(4-hydroxyl-2,2,6,6-tetramethylpiperidinyl-1-oxy),was explored for peptide-based thiyl radicals generated through the reaction between gluththione and peroxynitrite. Ac-Tempo·,a weak fluorescent compound,showed a dramatic increase in fluorescence intensity after interaction with thiyl radicals,and could be employed to probe peptide-based thiyl radicals. |
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| Keywords: | Spin-labeling Fluorescent probe Thiyl Radical Peroxynitrite |
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