HPLC波长切换法同时测定宝宝乐中四种成分的含量

目的建立HPLC波长切换法同时测定宝宝乐中芍药内酯苷、芍药苷、毛蕊异黄酮葡萄糖苷和芒柄花素的含量。方法采用Venusil MP C_(18)(250 mm×4.6 mm,5.0μm),以乙腈-0.1%磷酸溶液为流动相,进行梯度洗脱,检测波长分别为230 nm(芍药内酯苷、芍药苷)和254 nm(毛蕊异黄酮葡萄糖苷、芒柄花素),流速0.9mL/min,柱温30℃,进样量为10μL。结果芍药内酯苷、芍药苷、毛蕊异黄酮葡萄糖苷和芒柄花素4个成分的质量浓度分别在4.18~83.60μg/mL(r=0.999 8)、5.14~102.80μg/mL(r=0.999 5)、5.60~112.00μg/mL(r=0.999 9)、4.72~94.40μg/mL(r=0.999 3)范围内与峰面积呈良好的线性关系;平均加样回收率及相应的RSD分别为99.66%(0.96%)、98.17%(1.39%)、97.00%(1.48%)、99.92%(0.58%);精密度良好,RSD≤1.06%;重复性良好,RSD≤1.69%。供试品溶液在室温条件下12 h内稳定,RSD≤1.12%。结论所建立的方法灵敏度高、快速、专属性好、准确度高,为宝宝乐的质量控制提供了依据。

Simultaneous determination of the content of four constituents in Baobaole by HPLC wavelength switching method

Objective To develop an HPLC wavelength switching method for simultaneous determination of the content of alibiflorin,paeoniflorin,calycosin7-O-β-D-glucopyranoside and formononetin in Baobaole.Methods The Venusil MP C18 (250 mm × 4.6 mm,5.0 μm)chromatographic column was adopted;the mobile phase was acetonitrile-0.1％ phosphoric acid solution with gradient elution,at a flow rate of 0.9 mL/min.The detection wavelengths were 230 nm(determination of alibiflorin and paeoniflorin),and 254 nm(determination of calycosin7-O-β-D-glucopyranoside and formononetin).The column temperature was set at 30 ℃,and the sample quantity was 10 μL.Results The concentration of alibiflorin,paeoniflorin,calycosin7-O-β-D-glucopyranoside and formononetin was respectively 4.18～ 83.60 μg/mL(r =0.999 8),5.14～ 102.80 μg/mL (r =0.999 5),5.60 ～ 112.00 μg/mL (r =0.999 9) and 4.72 ～ 94.40 μ g/mL (r =0.999 3).The average recoveries and the corresponding RSD were 99.66％ (0.96％),98.17％ (1.39％),97.00％ (1.48％) and 99.92％ (0.58％),respectively.The precision was good,RSD≤ 1.06％;the repeatability was good RSD ≤ 1.69％.Test solution was stable at room temperature within 12 h,RSD ≤ 1.12％.Conclusion The established method is rapid,with high sensitivity,good specificity and high accuracy,which can be applied to the quality control of Baobaole.