基于角蛋白酶与蛋白酶协同处理的羊毛织物防毡缩加工

为提高羊毛酶法防毡缩整理效果，采用一种枯草芽孢菌产角蛋白酶和Savinase蛋白酶进行了羊毛酶处理实验，考察二步法协同处理对羊毛减量效果的影响。结果表明，单一角蛋白酶对羊毛减量效果较低，但能促进后续蛋白酶处理效率的提高。与氧化和蛋白酶处理相结合的防毡缩方法相比，经角蛋白酶、蛋白酶二步处理后毛织物的减量率与毡缩率略低，润湿性与染色性能有所改善，纤维损伤较少。羊毛氨基酸分析结果表明，角蛋白酶、蛋白酶处理后毛纤维中胱氨酸相对质量浓度低于氧化与蛋白酶处理样，验证了角蛋白酶能促使鳞片层中胱氨酸残基二硫键还原与蛋白变性，利于后续蛋白酶加工中毛纤维表面鳞片蛋白水解。

Enzymatic antifelting treatment of wool fabric with keratinase and protease

The goal of this study was to improve the effect of enzymatic antifelting treatment of wool via a novel keratinase from Bacillus subtilis and a Savinase protease. The influence of two-step enzymatic process on the weight loss of wool was investigated. The results indicated that keratinase treatment alone did not lead to obvious weight loss. However, it did promote the following protease treatment. In comparison with the conventional oxdition-protease process, the keratinase-cutinase process endowed wool fabrics with a bit lower weight loss, better wettability and dyeability as well as less fiber damage. The data from amino acid analysis indicated that the relative mass concentration of cystine for the keratinase-protease treated wool was lower than that of the oxidation-protease treated wool, which revealed that the keratinase treatment might promote the reduction of Cys–S-S-Cys in cuticle scales and make the succeeding protease treatment occur efficiently from the underlying protein layer of wool surface.