| 海金沙不同溶剂提取物清除自由基活性的研究 |
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| 引用本文： | 贲永光,李康,李坤平,张伟永.海金沙不同溶剂提取物清除自由基活性的研究[J].安徽农业科学,2009,35(19):8989-8991. |
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| 作者姓名： | 贲永光 李康 李坤平 张伟永 |
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| 作者单位： | 广东药学院药科学院,广东广州,510006 |
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| 基金项目： | 广东药学院博士启动基金 |
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| 摘 要： | 目的]比较不同溶剂提取海金沙总黄酮对自由基活性清除能力的大小。方法]应用DPPH法、邻苯三酚自氧化法和Fenton反应分别研究了浓度95％乙醇、乙酸乙酯、丙酮、乙酸、氯仿、甲醇提取物清除自由基活性的能力。结果]6种溶剂提取得到的海金沙提取物对DPPH·、OH·和O2^-·均有一定程度的清除作用，不同溶剂所得提取物对自由基的清除作用均有差别，其中浓度95％乙醇提取得到的海金沙提取物对3种自由基清除效果均最好。结论]海金沙可作为抗氧化物质资源进行开发利用。
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| 关 键 词： | 海金沙 黄酮 自由基 清除作用 |
Study on the Scavenging Activities of Extracts from Lygodium japonicum(Thunb)Sw. with Different Solvents to Free Radicals |
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| Affiliation: | BI Yong-guang et al ( College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006) |
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| Abstract: | Objective] The research aimed to compare the scavenging abilities of total flavone from Lygodium japonicum with different solvents to free radical activity.Method] DPPH method, pyrogallol method and Fenton reaction were used to study the scavenging ability of extracts with 95% ethanol, ethyl acetate, acetone, acetic acid, chloroform, methanol to free radical activity.Result] Six kinds of solvent extracts from L.japonicum had certain scavenging ability to DPPH·, OH · and O2-·.The scavenging ability of extracted with different solvents to free radicals were difference.The extracts from L.japonicum with 95% ethanol had the best scavenging effects on three kinds of free radicals.Conclusion] L.japonicum can be used as antioxidants for the development and utilization of resources. |
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| Keywords: | Lygodium japonicum(Thunb)Sw Flavone Free radicals Scavenging |
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