| 小鼠的非手术法采精 |
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| 引用本文： | 李聚学,郑新民,乔宪凤,周荆荣,魏庆信,曹斌云.小鼠的非手术法采精[J].动物学报,2004,50(4):657-661. |
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| 作者姓名： | 李聚学 郑新民 乔宪凤 周荆荣 魏庆信 曹斌云 |
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| 作者单位： | 1. 西北农林科技大学动物科技学院,陕西,杨凌,712100
2. 湖北省农业科学院生物技术研究所,武汉,430064 |
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| 基金项目： | 国家“8 63”计划项目 (No .2 0 0 1AA2 160 71)~~ |
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| 摘 要： | 用徒手法、假阴道抽吸法和电刺激法进行小鼠非手术法采精探索 ,结果表明 :假阴道抽吸法和电刺激法可采出精子 ,两者采精成功率、平均精子量和精子活率均差异不显著 ;小鼠不同射精阶段的精子含量不同 ,其中精子主要集中在射精的第二阶段和第三阶段 ;假阴道抽吸法和电刺激法对小鼠首次采精后 ,用同样的方法对相应的小鼠重复采精 ,成功率、平均精子量和精子活率与首次采精相比差异均不显著
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| 关 键 词： | 小鼠 非手术法采精 总精子数 精子活率 |
A method of mouse artificial spermiation |
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| LI Ju Xue ,ZHENG Xin Min ,QIAO Xian Feng ,ZHOU Jing Rong ,WEI Qing Xin ,CAO Bin Yun College of Animal Science and Technology,Northweastern Agriculture University,Yangling,Shanxi ,China, Biotechnology Institute,Hubei Academy of Agriculture Sciences,Wuhan ,China.A method of mouse artificial spermiation[J].Acta Zoologica Sinica,2004,50(4):657-661. |
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| Authors: | LI Ju Xue ZHENG Xin Min QIAO Xian Feng ZHOU Jing Rong WEI Qing Xin CAO Bin Yun College of Animal Science and Technology Northweastern Agriculture University Yangling Shanxi China Biotechnology Institute Hubei Academy of Agriculture Sciences Wuhan China |
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| Affiliation: | LI Ju Xue 1,ZHENG Xin Min 2**,QIAO Xian Feng 2,ZHOU Jing Rong 2,WEI Qing Xin 2,CAO Bin Yun 1 1 College of Animal Science and Technology,Northweastern Agriculture University,Yangling,Shanxi 712100,China, 2 Biotechnology Institute,Hubei Academy of Agriculture Sciences,Wuhan 430064,China |
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| Abstract: | Mouse artificial spermiation was studied by hand massage with artificial vagina and electrostimulation. The results show that artificial vagina and electro stimulation are achievable. The spermiation rate, average spermatozoa quantity and motility did not differ significantly between artificial vagina and electrostimulation. The spermatozoa quantity was different in each spermiation stage. The greatest quantity was in the second and third stage. |
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| Keywords: | Mouse Artificial spermiation Electrospermiation |
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