Intranucleolar localization of DNA topoisomerase IIalpha is a distinctive feature of necrotic, but not of apoptotic, Jurkat T-cells |
| |
Authors: | Falcieri Elisabetta Burattini Sabrina Bortul Roberta Luchetti Francesca Tabellini Giovanna Tazzari Pier Luigi Cappellini Alessandra Cocco Lucio Martelli Alberto M |
| |
Affiliation: | Istituto di Scienze Morfologiche, Campus Scientifico, Località Crocicchia, Università di Urbino, 61029 Urbino, Italy. e.falcieri@uniurb.it |
| |
Abstract: | Two distinct types of cell death have been described: apoptosis and necrosis. However, it is becoming increasingly clear that the differences between these two types are far less numerous than initially thought. Morphological analyses might provide important information to distinguish apoptotic from necrotic samples. We recently reported that in necrotic, but not apoptotic, HL-60 human myeloid leukaemia cells, the nuclear protein topoisomerase IIalpha concentrated in nucleoli. In order to ascertain whether or not this phenomenon was restricted to a peculiar cell type or could be detected also in cells of lymphoid lineage, we performed an investigation aimed at defining the localization of topoisomerase IIalpha in apoptotic and necrotic Jurkat human T lymphoblastoid cells. Immunofluorescence staining demonstrated that topoisomerase IIalpha was excluded from the condensed chromatin of apoptotic cells, whereas in necrotic cells it was localized in discrete nuclear dots. Immuno-electron microscopy analysis showed that topoisomerase IIalpha was undetectable in nucleoli of normal and apoptotic cells, whereas it was present in the nucleolus of necrotic cells irrespectively of the type of inducer used (ethanol, H(2)O(2), HgCl(2)). Taken together, our findings identify topoisomerase IIalpha as a potential morphological marker useful to discriminate between apoptotic and necrotic cells. |
| |
Keywords: | topoisomerase II α nucleolus changes immuno‐electron microscopy immunofluorescence diagnosis |
本文献已被 PubMed 等数据库收录! |
|