山核桃树叶中球松素查耳酮的含量测定

目的：建立用高效液相色谱法（HPLC）法测定山核桃树叶中球松素查耳酮（DHMC）含量的方法。方法：采用Kromasil C18（250 mm×4.6 mm,5μm）色谱柱,以甲醇-水（65∶35,V/V）为流动相,柱温为30℃,流速为1.0 ml/min,检测波长为342 nm,对DHMC的含量进行测定。结果：DHMC进样量在0.02～0.10μg范围内线性关系良好（r=0.999 6,n=6）,精密度RSD为1.1%（n=6）,重复性试验RSD为0.9%（n=6）,加样回收率为101.6%（RSD=1.8%,n=6）,经测定山核桃叶干品中DHMC的含量较高,达0.79%。结论：所建立的HPLC法专属性强,精密度好,结果准确。

Determination on the content of DHMC from the leaf of Carya cathayensis Sarg.

Objective： To establish the HPLC method for the determination on the content of DHMC in the Carya cathayensis Sarg..Methods： The Kromasil C18（250 mm×4.6 mm,5 μm） column was used for the determination on the content of DHMC with mobile phase consisted of methanol and water（65：35,v/v）.Column temperature was at 30℃,flow velocity was 1.0 ml/min,the detective wavelength was at 342 nm.Results： The standard curves of DHMC was linear in the concentration range of 0.02-0.10 μg（r=0.999 6,n=6）.The precision RSD was 1.1%（n=6）,the repeatability RSD was 0.9%（n=6）.The average recovery was 101.6%（RSD=1.8%,n=6）,the content of DHMC in Carya cathayensis Sarg.was 0.79%.Conclusion： This method is simple and accurate with good reproducibility.