| 抗黄曲霉毒素B1蛋黄抗体的制备及其性质的研究 |
| |
| 引用本文： | 陈福生,李根久,陈九武,罗信昌,周启.抗黄曲霉毒素B1蛋黄抗体的制备及其性质的研究[J].卫生研究,1999,28(4):241-243. |
| |
| 作者姓名： | 陈福生 李根久 陈九武 罗信昌 周启 |
| |
| 作者单位： | 华中农业大学农业微生物重点实验室,武汉,430070 |
| |
| 基金项目： | 湖北省科技厅科研项目，962P0810， |
| |
| 摘 要： | 将黄曲霉素Ｂ１－牛血清白蛋白（ＡＦＢ１Ｏ－ＢＳＡ）妆物免疫注射４只（Ａ、Ｂ、Ｃ、Ｄ）产蛋鸡。研究了抗ＡＦＢ１蛋黄抗体的产生进程，除鸡Ｂ外，其它３只鸡从第一次注射搞后的第９０天开始有较明显的抗体产生，第１３５天达到高峰，从第１６５天开始下降。在抗体产生高峰期，蛋黄抗体Ａ、Ｃ、Ｄ的间接ＥＬＩＳＡ效价分别为１：８０００、１：６０００、１：６０００。以蛋黄抗体Ａ为材料，研究了抗体的特异性，结果表明，该抗体
|
| 关 键 词： | 黄曲霉素素B1 抗体 产蛋鸡 蛋黄抗体 制备 ELISA |
Studies on the preparation and characters of anti-AFB1 antibody from the yolk of laying hens |
| |
| Chen Fusheng,Li Genjiu,Chen Jiuwu,Luo Xingchang,Zhou Qi.Studies on the preparation and characters of anti-AFB1 antibody from the yolk of laying hens[J].Journal of Hygiene Research,1999,28(4):241-243. |
| |
| Authors: | Chen Fusheng Li Genjiu Chen Jiuwu Luo Xingchang Zhou Qi |
| |
| Affiliation: | Agro-Microbiology Key Laboratories Huazhong Agricultural University, Wuhan 430070, China. |
| |
| Abstract: | AFB1O-BSA conjugates were injected into four laying hens (A, B, C and D). The yolk anti-AFB1 antibodies A,C and D produced were obviously increased after 90 days of the first injection, and reached the peak after 135 days, and started dropping after 165 days. The indirect ELISA titer of yolk antibodies A,C and D were 1:8000, 1:6000 and 1:6000 respectively. The specificity of the antibody A was good, but a cross-reaction with AFB2, AFG1, AFG2, with an exception of AFM1, was found. The sensitivities of AFB1, AFB2, AFG1 and AFG2 were 6, 25, 125 and 2495 micrograms/L respectively. |
| |
| Keywords: | |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
