反相高效液相色谱法测定香青兰中槲皮素的含量

目的：建立测定香青兰中槲皮素含量的方法。方法：色谱柱为Kromasil C18（4．6×150mm，5um），流动相为乙腈-5％甲酸（31：69），检测波长：280nm，流速：1.0mL·min^-1，柱温：30℃。结果：香青兰中槲皮素的线性关系良好，其线性范围为0．02mg·mL^-1～0．10mg·mL^-1（r=0．9990）。其回收率为97．2％（RSD=1．2％）。结论：所建方法准确、简便，为科学评价香青兰的质量提供了依据。

Determination of Quercetin content in Dracoephalum mololariea L by RP-HPLC

Objective： To establish a method for determination of the content of quercetin in Dracoephalum mololariea L. Metheds： Kromasil C18 （4. 6 × 150mm, Sum） were used, Acetonitrile-5% Formic acid （31： 69） was served as mobile phase, and flow rate was 1.0ml · min^-1, wavelength for determine was 280 nm. Results： The calibration curves were linear in the range of 0. 02mg · mL^-1 -0. 10mg · mL^-1 （r=0. 9990） , The average recovery rates with the RSD were 97. 2 % （ 1.2% ） , respectively. Conclusion ： The method is accurate, and suitable for the determination of the content of quercetin in Dracoephalum mololariea L. The method can be adopted for quality control of Dracoephalum mololariea L.