泽泻汤组分不同配比对豚鼠膜迷路积水影响的比较

目的:观察泽泻汤中泽泻与白术不同剂量配比对膜迷路积水豚鼠的影响。方法:采用腹腔注射醋酸去氨加压素法复制膜迷路积水动物模型。观察各组豚鼠的行为学变化及蜗管组织病理学改变,计算各组豚鼠蜗管横截面积与蜗管加前庭面积之和(总面积)的比值,以评价膜迷路积水的程度。结果:与空白组比较,模型组豚鼠蜗管横截面积与总面积的比值明显升高(P<0.05);与模型组比较,3组实验组豚鼠蜗管横截面积与总面积的比值均下降,但实验1组与模型组比较差异有统计学意义(P<0.05)。结论:3组实验组中,以原方剂量配比(泽泻∶白术为5∶2)的实验1组缓解豚鼠膜迷路积水作用最佳,证实了仲景方剂配伍的科学合理性。

Effects Comparison Among Different Ratio of Zexie and Baizhu in Zexie Decoction on Guinea Pigs With Endolymphatic Hydrops

Objective：To observe effects of zexie decoction with different ratio of zexie and baizhu on endolymphatic hydrops in guinea pigs.Methods：Labyrinthin hydrops guinea pig models were established with intraperitoneal injection of desmopressin acetate.Behavipral changes of guinea pigs and cochlear duct changes of pathohistology were observed.Ratio of cross-sectional area and total area（cochlear and vestibular area）was calculated.Result：Compared with blank control group,ratio of cross-sectional area and total area was increased in model group（P0.05）;ratios of cross-sectional area and total area in 3 experimental groups were lower than that in model group The difference between model group and the first experimental group was statistically significant（P0.05）.Conclusion：Effect of the first experimental group（zexie∶baizhu=5∶2）on endolymphatic hydrops is the best among 3 experimental groups.