| 基于聚硫堇的一次性O型口蹄疫抗原酶免疫传感器的研制 |
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| 引用本文： | 丁建英,韩剑众,顾春海,冯志梅. 基于聚硫堇的一次性O型口蹄疫抗原酶免疫传感器的研制[J]. 传感技术学报, 2010, 23(12): 1673-1678. DOI: 10.3969/j.issn.1004-1699.2010.12.001 |
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| 作者姓名： | 丁建英 韩剑众 顾春海 冯志梅 |
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| 作者单位： | 常熟理工学院生物与食品工程学院,江苏,常熟,215500;浙江工商大学食品质量与安全重点实验室,杭州,310035;浙江工商大学食品质量与安全重点实验室,杭州,310035;常熟理工学院生物与食品工程学院,江苏,常熟,215500 |
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| 基金项目： | 浙江省“重中之重”项目子项目资助(ZZ05-07) |
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| 摘 要： | 将O型口蹄疫酶标抗体掺杂于有机-无机溶胶凝胶中,并修饰于聚硫菫的丝网印刷碳电极表面,从而制备一次性口蹄疫病毒抗原(FMDV-Ag)酶免疫传感器。试验中采用直接免疫分析法检测口蹄疫病毒抗原。根据免疫反应前后还原峰电流下降的百分率K值的大小实现对抗原的检测。在优化条件下,免疫传感器检测抗原的线性范围为77～388ng/mL,最低检出限为32ng/mL。免疫电极具有较好的特异性、重现性(RSD=5.6%)、稳定性(10d后电流响应为初始值的89.5%)和准确性(与AGP符合率为95%)。因此,该免疫传感器有望用于O型口蹄疫抗原的快速检测。
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| 关 键 词： | 电化学免疫传感器 O型口蹄疫抗原快速检测 丝网印刷碳电极 聚硫堇 |
Fabrication of Enzyme Immunosensor for Detection of typeO FMDVAg Based On Poly( thionine) modified Screen-printed Carbon Electrode |
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| DING Jianying,HAN Jianzhong,GU Chunhai,FENG Zhimei. Fabrication of Enzyme Immunosensor for Detection of typeO FMDVAg Based On Poly( thionine) modified Screen-printed Carbon Electrode[J]. Journal of Transduction Technology, 2010, 23(12): 1673-1678. DOI: 10.3969/j.issn.1004-1699.2010.12.001 |
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| Authors: | DING Jianying HAN Jianzhong GU Chunhai FENG Zhimei |
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| Affiliation: | DING Jianying1,2,HAN Jianzhong2,GU Chunhai1,FENG Zhimei1 1.College of Biotechnology , food Engineering,Changshu Institute of Technology,Changshu Jiangsu 215500,China,2.Zhejiang Gongshang University,Food Safety Key Lab of Zhejiang Province,Hangzhou 310035 |
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| Abstract: | A disposable electrochemical immunosensor for detection of FMDV based on the poly(thionine) modified screen-printed electrode coated with Chitosan-silica sol-gel doped with HRP-anti-FMDV has been developed in the experiment.Then the immunosensor was characterized by cyclic voltammetry(CV).According to the decrease percentage of response current,type-O FMDV-Ag could be detected.Under the optimum conditions,type-O FMDV-Ag can be rapidly detected by sigmoidal curve method and form a linear calibration between ... |
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| Keywords: | electromical immunsensor rapid detection of FMDV-Ag(antigen of foot and mouth disease virus) screen-printed carbon electrode polymerization of thionine |
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