灯盏花素小肠吸收影响因素的研究

目的观察灯盏花素在体小肠吸收的影响因素。方法应用大鼠在体肠循环单向灌流法,以高效液相色谱（HPLC）法测定灯盏花素中灯盏乙素含量,研究灯盏花素在小肠不同肠段、不同pH、不同浓度下的吸收情况,并考察转运蛋白对药物小肠吸收的影响。结果：灯盏花素在不同质量浓度下,未发现饱和现象,吸收速率基本保持不变;在pH6.0-7.4条件下,灯盏花素的小肠吸收不受pH的影响;不同小肠部位对其吸收速率基本相同;加入P-糖蛋白（Pgp）抑制剂后药物吸收率没有明显变化;加入多药耐药相关蛋白2（MRP2）抑制剂后药物吸收率显著增加。结论：灯盏花素在整个肠段均有吸收;其吸收机制为被动扩散;P-gp对灯盏花素的小肠吸收基本没有影响,而MRP2可降低灯盏花素的吸收。

Study on Influence Factors of Intestinal Absorption of Breviscapine

Objective To study the influence factors of Breviscapine intestinal in rat. Methods Rat single pass intestinal perfusion was used to study breviseapine intestinal absorption, HPLC was employed to determined Breviscapinecontent, research intestinal absorption of breviscapine in different intestinal segments, different pH and different concentrations of the drug transport proteins. Results No saturation phenomenon occurred at different concentrations of breviseapine ; the absorption of breviscapine was not influenced by pH values ranging from 6.0 to 7.4; the absorption of breviseapine was not influenced by different part of intestine;after addition to P -gp inhibitor, the intestinal ab- sorption of breviscapine has no change; while for MRP2 inhibitor, absorbing power were significantly higher. Conclusion Breviscapine can be absorbed by all intestine. The absorption of breviscapine complied with the passive diffusion mechanism. P - gp transporter has no effect on intestinal absorption of breviscapine, and MRP2 transporter can decrease the intestinal absorption of breviscapine.