六月青多糖对鸭乙型肝炎病毒诱导肝损伤的保护作用研究

目的:观察六月青多糖(LYQP)对鸭乙型肝炎所致肝损伤的保护作用,为研究和开发新的抗乙型肝炎病毒(HBV)药物提供实验依据.方法:采用鸭乙型肝炎病毒(DHBV)诱导广西麻鸭乙型肝炎病毒肝损伤模型.分别检测给药前(T0)、给药7 d(T7),14 d(T14)及停药后3d(P3)血清中丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)的活性,同时采用酶联免疫吸附( ELISA)法测定上清液鸭乙型肝炎病毒表面抗原(DHBsAg)和e抗原(DHBeAg)的滴度,HE染色观察肝损伤程度.结果:与模型组相比,各给药组鸭血清ALT,AST的活性及血清DHBsAg,DHBeAg的滴度显著降低(P＜0.05或P＜0.01);停药3d后,LYQP高、中剂量组血清ALT,AST活性及血清DHBsAg,DHBeAg的滴度均无反跳现象.HE染色结果显示LYQP可显著减轻鸭肝组织病理损伤程度.结论:LYQP对DHBV所致肝损伤具有保护作用.

Protective Effect of Liuyueqing Polysaccharide against Duck Liver Injury Induced by Duck Hepatitis B Virus

Objective: To study the protective effect of traditional Chinese medicine Liuyueqing polysaccharide(LYQP) on duck liver injury induced by duck hepatitis B virus(DHBV),and provide an experimental base for research and developing a new durg for anti-HBV. Method: Liver injury induced by DHBV in Guangxi brown spotted ducks was used as animal model. Alanine aminotransferase(ALT), aspartate aminotransferase(AST), DHBsAg and DHBeAg in serum were detected before treatment(T₀), 7 days(T₇), 14 days(T₁₄)after treatment, and 3 days after stopping treatment.The extent of liver injury was observed by HE staining. Result: Compared with modle group, the serum levels of ALT, AST, DHBsAg and DHBeAg were decreased significantly (P<0.05 or P<0.01) and at 3 days after stopping treatment, the ALT, AST, DHBsAg and DHBeAg in serum in high-dose and middle-dose did not return. HE staining showed that LYQP had significant protective effects against the duck liver injury induced by DHBV. Conclusion: LYQP has a protective effect against the duck liver injury induced by DHBV.