| 用两种方法检测EGF对肝细胞DNA合成影响的比较 |
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| 引用本文： | 陈图兴,孙宏训.用两种方法检测EGF对肝细胞DNA合成影响的比较[J].南京医科大学学报,1991(4). |
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| 作者姓名： | 陈图兴 孙宏训 |
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| 作者单位： | 南京医学院第一附属医院内科
(陈图兴)，南京医学院第一附属医院内科(孙宏训) |
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| 摘 要： | 分别应用单克隆抗BrdU和~3H胸腺嘧啶标记DNA方法检测了表皮生长因子对肝细胞DNA合成的影响,并对两种方法进行了比较。结果显示,两种方法均证明EGF具有明显的刺激肝细胞DNA合成的作用。单克隆抗BrdU标记DNA方法较之~3H胸腺嘧啶法更特异、准确,可以清楚地区分DNA合成期与非合成期细胞。同时简便、完全和省时。
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| 关 键 词： | 抗体 单克隆 单克隆抗溴脱氧尿嘧啶抗体 胸腺嘧啶 细胞计数 表皮生长因子 脱氧核糖核酸 细胞 肝 |
Comparison of Two Methods for Detecting the Effect of EGF on DNA Synthesis of Hepatocystes |
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| Chen Tuxing,Sun Hongxun.Comparison of Two Methods for Detecting the Effect of EGF on DNA Synthesis of Hepatocystes[J].Acta Universitatis Medicinalis Nanjing,1991(4). |
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| Authors: | Chen Tuxing Sun Hongxun |
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| Affiliation: | Chen Tuxing;Sun Hongxun |
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| Abstract: | The effect of EGF on DNA synthesis of rat hepatocytes was detected usingmonoclonal anti-bromodeoxyuridine anti-body and ~3H-thymidine, and the two methods werecompared. The results of both methods confirmed that EGF could substantially stimulateDNA synthesis of hepatocytes. Anti-BrdU staining was more specific, accurate and clearerfor differentiating DNA-synthesizing cells from non DNA-synthesizing ones than ~3H-thymidine technique. It has the advantages of simple and safe handling and a comparativelyshort processing time. |
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| Keywords: | antibodies monoclonal monoclonal anti-bromodeoxyuridine (anti-BrdU) thymidine cell count epidermal growth factor DNA cells liver |
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