血链球菌血链素的分离纯化及其化学组成的测定

目的:分离纯化血链球菌血链素,并对其组成成分进行检测。方法:通过羟基磷灰石(HA)柱层析、SephadexG-150凝胶柱层析、中空纤维柱超滤脱盐、浓缩,分离并纯化血链素,SDS-PAGE检测其纯度及亚基分子量并检测其分子量,同时以紫外线吸收法及苯酚-硫酸法检测血链素蛋白及糖含量。结果:血链素分子量为180kDa,亚基分子量为110kDa和70kDa,其中蛋白含量为87.51%,糖含量为12.49%。结论:纯化的血链素是一种由两个亚基组成的糖蛋白。

Isolation, purification, and chemical composition of sanguicin from Streptococcus sanguis

Objective:To isolate and purify sanguicin from Streptococcus sanguis and determine its chemical composition.Methods:Sanguicin was obtained from the culture supernatant by using hydroxylapatite column chromatography,Sephadex G-150 gel chromatography,and ultra-filtration.The purity,molecular weight,and subunit molecular weight of sanguicin were determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis.The proportions of protein and saccharide were determined by ultraviolet absorption technique and phenol-sulfuric acid method,respectively.Results:The molecular weight of sanguicin was 180 kDa,and the molecular weights of 2 subunits of sanguicin were 110 and 70 kDa,respectively.The proportions of protein and saccharide were 87.51% and 12.49%.Conclusion:Sanguicin from Streptococcus sanguis is a glycoprotein which consists of 2 subunits.