猪轮状病毒与猪传染性胃肠炎病毒混合感染的实验室诊断

[目的]对海南省某猪场腹泻病死仔猪病原进行实验室诊断。[方法]通过临床症状观察与病理剖检,结合RT-PCR方法和病毒的分离培养进行病原鉴定。[结果]经过RT-PCR扩增,获得了PRV和TGEV阳性目的片段;应用MA-104细胞与PK-15细胞分别进行PRV与TGEV的分离培养后,可见典型的细胞病变,经过RT-PCR进一步鉴定最终确诊为PRV和TEGV混合感染。[结论]通过采用紧急接种和保守治疗等综合防治措施,获得了较好的防控效果。

Laboratory Diagnosis of Mixed Infections of Porcine Rotavirus and Transmissible Gastroenteritis Virus

[Objective] To make laboratory diagnosis of dead pigs from a hoggery of Hainan Province.[Method] By using the clinical examination,pathologic autopsy,RT-PCR and cell culture methods,the pathogen of dead pigs were separated and identified.[Result] The positive fragments of porcine rotavirus(PRV)and transmissible gastroenteritis virus (TGEV)were obtained by RT-PCR.After PRV and TGEV were isolated and cultured in MA-104 cells and PK-15 cells respectively,the typical cytopathic effect displayed.The mixed infection of PRV and TGEV was further identified by RT-PCR.[Conclusion] Better curative effects were obtained after comprehensive treatment measures such as emergent inoculation and conservative treatment were taken.