小麦胚芽油抗动脉粥样硬化的药效学研究

目的：观察小麦胚芽油（wheat germ oil，WGO）对动脉粥样硬化（atherosclerosis，AS）大鼠的治疗效果，并探讨其抗 AS 的可能作用机制。方法连续高脂乳剂灌胃4周，制作 AS 大鼠模型，分别给予各组大鼠连续灌胃不同剂量 WGO 8周；HE 染色光镜下观察大鼠主动脉弓病理形态的改变；酶联免疫吸附试验（ELISA）法检测各组大鼠血清中前列环素（Prostacyclin 2， PGI2）、血栓素 A2（Thromboxane 2，TXA2）及内皮素-1（Endothelin-1，ET-1）的含量。结果2．0 g·kg －1的 WGO 能明显改善 AS大鼠主动脉弓的病理状态，基本恢复正常状态；与模型组比较，1．0、2．0 g·kg －1 WGO 均可显著提高 AS 大鼠血清中 PGI2含量，明显降低 TXA2、ET-1水平（P ＜0．05）。结论WGO 能明显改善 AS 大鼠的动脉粥样硬化状态。其作用机制可能为小麦胚芽油具有良好的调节 PGI2、TXA2平衡，抑制血栓形成，修复内皮损伤以及较强的抗氧化作用。

Pharmacodynamic effects of wheat germ oil on atherosclerosis

Objective To investigate the effects of wheat germ oil on atherosclerosis in rats and to explore its possible mechanism. Methods Model of AS rats was made by intragastric administration of fat emulsion for four weeks consecutively.Different doses of WGO were given to the rats which were divided into different groups by intragastric administration for 8 weeks.Pathomorphological changes in aortic arch of AS rats were observed by light microscopy (HE staining).Enzyme-Linked Immunosorbent Assay was used to evaluate the sample concentrations of PGI2,TXA2 and ET-1 in rats'' serum. Results The pathomorphological abnormalities of AS rats'' aortic arch were significantly improved in high dose group of wheat germ oil,which returned to normal state;WGO could also apparently increase PGI2 and reduced TXA2,ET-1 levels (P <0.05). Conclusions Wheat germ oil can significantly improve the state of atherosclerosis in rats.The mechanism may refer to its good ability to regulate the balance between PGI2 and TXA2,inhibit thrombosis and endothelial damage as well as its strong antioxidant effect.