| 高三尖杉酯碱对Jurkat细胞端粒酶活性的影响及机制研究 |
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| 引用本文： | 王蕾,金洁. 高三尖杉酯碱对Jurkat细胞端粒酶活性的影响及机制研究[J]. 实用肿瘤杂志, 2005, 20(5): 391-394 |
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| 作者姓名： | 王蕾 金洁 |
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| 作者单位： | 浙江大学医学院附属第一医院血液科,浙江,杭州,310003 |
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| 基金项目： | 浙江省自然科学基金项目（300507） |
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| 摘 要： | 目的 探讨高三尖杉酯碱（HHT）对人T淋巴细胞白血病细胞株Jurkat细胞端粒酶活性的影响及其机制.方法 采用改良的Krupp荧光素标记的端粒重复扩增（TRAP）方法检测HHT对Jurkat细胞端粒酶活性的影响.半定量RT-PCR方法检测HHT作用后Jurkat细胞端粒酶逆转录酶（hTERT）及c-myc原癌基因mRNA的表达水平.结果 HHT对Jurkat细胞端粒酶活性及hTERTmRNA、c-myc基因mRNA表达有明显抑制作用.结论 HHT可通过下调hTERT基因转录抑制淋巴细胞的端粒酶活性.c-myc可能参与调控淋巴细胞hTERT基因转录.
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| 关 键 词： | 白血病 T细胞 急性 高三尖杉酯碱 端粒酶 RNA聚合酶类 聚合酶链反应 原癌基因蛋白质c-myc 细胞凋亡 |
| 文章编号： | 1001-1692（2005）05-0391-04 |
| 收稿时间： | 2003-05-19 |
| 修稿时间： | 2003-05-19 |
Study on inhibition of telomerase activity of human T lymphocyte Jurkat cells by homoharringtonine and its mechanism |
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| WANG Lei,JIN Jie. Study on inhibition of telomerase activity of human T lymphocyte Jurkat cells by homoharringtonine and its mechanism[J]. Journal of Practical Oncology, 2005, 20(5): 391-394 |
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| Authors: | WANG Lei JIN Jie |
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| Affiliation: | Department of Hematology, First Affiliated Hospital ,Cellege of Medicine ,Zhejiang University ,Hangzhou, 310003 ,China |
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| Abstract: | Objective To investigate the effect and mechanism of homoharringtonine on telomerase activity of human T lymphocyte Jurkat cells. Methods The telomerase activity of Jurkat cells was examined by modified Krupp TRAP assay. The expression of c-myc and hTERT mRNA was half-quantified using RT-PCR assay. Results Telomerase activity was significantly inhibited by HHT. Simultaneously,the down-regulation of hTERT mRNA and c-myc mRNA expressions was observed in Jurkat cells treated with HHT. Conclusion The inhibition of telomerase activity by HHT is due to the repressed hTERT mRNA expression, c-myc gene might regulate the transactivation of hTERT gene in the cells. |
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| Keywords: | leukemia, T-cell, acute homoharringtonine telomerase RNA polymerases polymerase chain reaction proto-oncogene proteins c-myc apoptosis |
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