Enzyme-linked immunosorbent assays for the insecticide fenitrothion. Influence of hapten conformation and sample matrix on assay performance |
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Authors: | Kim Yoo Jung Kim Young Ah Lee Yong Tae Lee Hye-Sung |
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Affiliation: | a Department of Food Science and Nutrition, Kyungpook National University, Daegu 702-701, Republic of Korea b Department of Biochemistry, Yeungnam University, Gyongsan 712-749, Republic of Korea |
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Abstract: | This study aimed at developing competitive enzyme-linked immunosorbent assays (ELISAs) for the organophosphorus (OP) insecticide fenitrothion using a monoclonal antibody. The hapten used to obtain the antibody had an ideal structural feature that allowed minimal functional group sacrifice. By using the antibody and a coating antigen, a competitive indirect ELISA was developed, which showed an IC50 of 14 ng mL−1 with a detection limit of 3.0 ng mL−1. A competitive direct ELISA using an enzyme tracer was also developed, which showed an IC50 of 17 ng mL−1 with a detection limit of 1.6 ng mL−1. The antibodies in both assays showed negligible cross-reactivity with the metabolites of fenitrothion and other OP pesticides except with the insecticides parathion-methyl and parathion-ethyl. Recoveries of fenitrothion from fortified rice and lettuce samples were determined and the bias in the recovery values was rationalized by using the standard curves obtained in the matrix extract. |
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Keywords: | Fenitrothion Insecticide Monoclonal antibody Immunoassay Enzyme-linked immunosorbent assay |
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