一株秸秆纤维素降解菌株的筛选及其降解特性

纤维素是自然界一种最重要的可再生资源,从玉米地土壤中分离出1株产纤维素酶能力较强的菌株J-N3.以羧甲基纤维素为唯一碳源的分离培养基和刚果红染色法进行初筛,再将筛选到的菌株进行液体发酵培养并测定酶活.结合形态学特征与分子生物学鉴定结果得知,菌株J-N3为芽孢杆菌属(Bacil-lus).并对其酶学性质进行初步研究,得到...

Screening of a Cellulose-degrading Strain and its Core Degrading Effect on Corn Stalk

Cellulose is one of the most important renewable resources in nature. In this paper, a strain j-n3 was isolated from the soil of corn field. The isolated medium with carboxymethyl cellulose as the sole carbon source and Congo red staining method were used for preliminary screening, and then the screened strains were cultured by liquid fermentation and enzyme activity was determined. Combined with morphological characteristics and molecular biological identification, strain j-n3 was identified as Bacillus.According to the preliminary study of enzymatic properties, the optimum pH of the enzyme is 6.0, and the optimum reaction temperature is 50℃. After treated with strain J-N3 for 20 days, the weight loss rate and cellulose decomposition rate of corn stalks reached 26.22% and 31.01%, respectively.