三种不同材料注射构建高眼压模型兔的比较

背景:目前对青光眼的发病机制、过程、损害的研究很多,但缺乏理想的青光眼模型.目的:利用不同材料制作3种兔高眼压动物模型,并验证各种兔高眼压动物模型在青光眼研究以及临床中实用价值.方法:选普通级健康新西兰白兔30只,随机数字表法把30只兔子随机分为3组,每组10只.将每只兔子的一眼作为实验眼,另一只眼作为对照眼.3组实验眼前房内分别注射自体血、甲基纤维素、长效气体C3F8材料;对照眼用同样的方法注射生理盐水,建立青光眼动物模型.记录注射前、注射后当时、24,36,48,72,96,120,168 h眼压变化.结果与结论:三种材料均可诱发实验兔成为高眼压模型,3组兔子眼压均有明显升高(P＜0 05),其升高的幅度及维持的时间不同.3种材料诱发高眼压3持续时间分别为1,3,7 d,重复上述注射眼压可再次升高,维持高眼压时间更久.3组动物眼压波动在1.86～6.65 kPa之间.眼前节出现轻度炎症反应.证实利用自血、甲基纤维素、C3F83种材料建立的3种兔眼模型均是理想的高眼压模型,具有方法简单,易于操作和控制等特点.依据研究的需要,可以选择不同的实验动物模型,可用于急、慢性青光眼的相关研究.

Comparison of rabbit intraocular hypertension models prepared by three different material injections

BACKGROUND: Currently, there are many studies concerning the pathogenesis, process, and damage of glaucoma, however, there is not an ideal glaucoma model. OBJECTIVE: To prepare rabbit intraocular hypertension models using three different material injections, and to verify the practical value of intraocular hypertension models. METHODS: Thirty New Zealand rabbits were divided randomly into 3 groups, with 10 animals in each group. One eye of each rabbit was served as the experimental eyes and the other eye as...