c-erbB-2、VEGF和组织蛋白酶D在胃癌中的表达及其相关性

目的：探讨癌基因c-erbB-2、血管内皮生长因子（VEGF）和组织蛋白酶D（Cath-D)在胃癌中的表达及其与胃癌生物学行为的关系。方法：采用免疫组织化学（S－P）法，检测了102例胃癌手术标本中c-erbB-2、VEGF及Cath-D的表达，同时观察了网状纤维分布与c-erbB-2、Cath-D之间的关系，并将检测结果与跟踪随访资料进行了综合分析。结果：102例胃癌组织中c-erbB-2表达阳性率为38.24%（39／102），与胃癌浸润深度（P＜0.05）、淋巴结转移（P＜0.05）密切相关；VEGF表达阳性率为50％（51／102），与胃癌浸润深度（P＜0.01）、生长方式（P＜0.01）、淋巴结转移（P＜0.01）密切相关；Cath-D表达阳性率为81.37%(83/102)，与胃癌浸润深度（P＜0.05）、生长方式（P＜0.05）、淋巴结转移（P＜0.05）及脉管内有无癌栓（P＜0.05）有关。对生存期分析显示：Cath-D、VEGF、c-erbB-2阳性患者预后差，5年生存率低于阴性表达患者。结论：c-erbB-2、VEGF及Cath-D与胃癌的生长、浸润、转移、预后有密切关系，可作为判断胃癌生物学行为和预后的重要指标。

Expression of c-erbB-2,VEGF and cathepsin D in gastric carcinoma and their relationship

Purpose To investigate the expression of c erbB 2 oncogene ,VEGF and cathepsin D(Cath D) in gastric carcinoma and their correlation with the biological behavior of gastric carcinoma (GC). Methods The expression was studied in 102 cases of GC by immunohistochemical technique. Meanwhile, the correlation of c erbB 2,VEGF,Cath D and reticular fiber staining in GC were studied. Results Thirty nine cases of the gastric carcinoma specimens (38 24%) were positive for c erbB 2 and correlated with depth of invasion ( P< 0 05) and lymph node metastasis ( P< 0 05); Fifty one case of the gastric carcinoma specimens (50%) were positive for VEGF and correlated with depth of invasion ( P< 0 01), growth pattern ( P< 0 01) and lymph node metastasis ( P< 0 01); eighty three cases of the 102 gastric carcinoma specimens (81 37%) were positive for Cath D and correlated with depth of invasion ( P< 0 05), growth pattern ( P< 0 05), lymph node metastasis ( P< 0 05) and blood vessel cancer embolus ( P< 0 05). Patient survival analysis showed that the prognosis of patients with gastric carcinoma with positive expression of c erbB 2, VEGF or Cath D was poorer. The five years survival rate was significantly lower in gastric carcinoma patients with positive expression of c erbB 2,VEGF or Cath D. Conclusion The results suggest that expression of c erbB 2 and Cath D is closely related to growth, invasion, metastasis and prognosis of gastric carcinoma.