| 双水相体系纯化天冬氨酸酶、纤维素酶和脂肪酶 |
| |
| 引用本文： | 阎佰旭,靳小红,杨红,马林,冯雁,曹淑桂,程玉华.双水相体系纯化天冬氨酸酶、纤维素酶和脂肪酶[J].吉林大学学报(理学版),1991(4). |
| |
| 作者姓名： | 阎佰旭 靳小红 杨红 马林 冯雁 曹淑桂 程玉华 |
| |
| 作者单位： | 吉林大学酶工程实验室 长春
(阎佰旭，靳小红，杨红，马林，冯雁，曹淑桂)，吉林大学酶工程实验室 长春(程玉华) |
| |
| 摘 要： | 用聚乙二醇(PEG)/磷酸钾双水相抽提技术分别从天冬氨酸粗酶、商品纤维素酶和脂肪酶中分离纯化出天冬氨酸酶(Aspartase)、纤维素酶(Cellulase)和脂肪酶(Lipase)。研究了PEG分子量及浓度、磷酸钾浓度、蛋白质含量、pH、氯化钠浓度等因素对分配系数,酶活回收率和分离效果等参数的影响,并设计出双水相体系抽提三种酶的相组成系统。
|
| 关 键 词： | 双水相体系 相组成系统 天冬氨酸酶 纤维素酶 脂肪酶 |
Purification of Aspartase, Cellulase and Lipase by Aqueous Two-phase |
| |
| Yan Baixu,Jin Xiaohong,Yang Hong,Ma Lin,Feng Yan,Cao Shugui and Cheng Yuhua.Purification of Aspartase, Cellulase and Lipase by Aqueous Two-phase[J].Journal of Jilin University: Sci Ed,1991(4). |
| |
| Authors: | Yan Baixu Jin Xiaohong Yang Hong Ma Lin Feng Yan Cao Shugui and Cheng Yuhua |
| |
| Abstract: | The influences of molecular weight of PEG, concentrations of PEG and potassium phosphate, the amounts of protein and concentration efficiency were studied. The optimum conditions of aqueous two-phase systems for separating aspartase, cellulase and lipase from the crude aspartase, industrial cellulase and lipase respectively are devised. |
| |
| Keywords: | aqueous two-phase system compositions of aqueous two-phase system aspartase cellulase lipase |
| 本文献已被 CNKI 等数据库收录！ |
|
