几种抗生素与人血清白蛋白结合反应的研究

用荧光法研究了头孢噻肟钠、苯唑西林、阿莫西林、诺氟沙星、依诺沙星等五种抗生素类药物与人血清白蛋白的结合反应,测得26℃时的结合常数K_A分别为1.98×10⁴,1.01×10³,1.38×10³,5.97×10⁴和7.15×10⁴L·mol^-1,结合位点数n分别为1.16,0.86,1.19,0.91和0.93.确定了这些药物与人血清白蛋白之间的主要结合力为静电作用力.

Studies on the Binding Mechanism of Several Antibiotics and Human Serum Albumin

The binding characteristics of antibiotics, cefotaxime sodium, oxacillin, amoxicillin, norfloxacin and enoxacin with human serum albumin (HSA) were studied by fluorescence spectrometry. These drugs quenched the HSA fluorescence via a nonradiative energy transfer mechanism. The binding constants (K_A) and binding sites (n) for drug-HSA were obtained by an improved analysis method to process the experimental data. The values of K_A obtained were 1.98×10⁴, 1.01×10³, 1.38×10³, 5.97×10⁴ and 7.15×10⁴ L?mol^－1 for cefotaxime sodium-HSA, oxacillin-HSA, amoxicillin-HSA, norfloxacin-HSA and enoxacin-HSA, respectively, at 26 ℃. The values of n were 1.16, 0.86, 1.19, 0.91 and 0.93, respectively, for these drugs in the above order. The electrostatic interaction was found to be main acting force in the studied systems.