PCNA、Caspase-3在翼状胬肉中的表达与细胞增殖的关系

目的:探讨PCNA、Caspase-3在翼状胬肉中的表达与细胞增殖及发病过程中的作用。方法:采用免疫组化Elivision法检测20例翼状胬肉、10例正常结膜中PCNA、Caspase-3的表达。结果:20例翼状胬肉中Caspase-3的阳性表达率为55%(11/20),正常结膜中Caspase-3的阳性表达率为90%(9/10)。PCNA在翼状胬肉中的阳性表达率为95%(19/20),正常结膜中PCNA的阳性表达率为60%(6/10)。Caspase-3、PCNA阳性表达两组间差异有显著意义(P<0.05)。结论:PCNA在翼状胬肉中的高表达和Caspase-3在翼状胬肉中的低表达,提示细胞凋亡和增殖异常,可能与翼状胬肉的发生、发展有关。

Relation of Expression of PCNA,Caspase-3 and Cell Proliferation in Pterygium

Objective To study the expressions and its significance of proliferating cell nuclear antigen(PCNA) and Caspase-3 in human pterygium.Methods The immunohistochemical Elivision method was used to examine the expressions of PCNA and Caspase-3 in 20 cases of pterygium and 10 cases normal conjunctiva.Results The positive rate of Caspase-3 was 55.0% in pterygium and was 90% in the normal conjunctive.The positive rate of PCNA was 95.0% in pterygium and was 60% in the normal conjunctive.There was significant difference in the expressions of Caspase-3 and PCNA between the pterygium and normal conjunctive(P<0.05).Conclusion There is a low Caspase-3 expressions and a high PCNA expression in pterygium which suggest that there is abnormal apoptosis and proliferation and that the PCNA and caspase-3 plays an important role in incidence and development of pterygium.