| 碱茅Put-HVA1基因的克隆及在酵母和拟南芥中的表达 |
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| 引用本文： | 刘小立,柳参奎,高野哲夫.碱茅Put-HVA1基因的克隆及在酵母和拟南芥中的表达[J].分子植物育种,2010,8(5). |
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| 作者姓名： | 刘小立 柳参奎 高野哲夫 |
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| 作者单位： | 1. 东北林业大学盐碱地生物资源环境研究中心,哈尔滨,150040
2. 东京大学亚洲生物资源环境研究中心,东京,1880002,日本 |
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| 基金项目： | 国家林业局948项目，黑龙江省杰出青年科学基金 |
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| 摘 要： | 本研究从碱茅(Puccinellia tenuiflora)cDNA文库中分离得到Put-HVA1基因,其开放读码框(ORF)长534bp,共编码177个氨基酸,其预测分子量约为18.167kD,理论等电点约为7.80。氨基酸序列比较结果表明,Put-HVA1氨基酸序列与水稻、玉米的HVA1蛋白氨基酸序列的同源性分别为69%和56%。另外,将Put-HVA1基因构建到酵母表达载体pYES2和植物双元表达载体pBI121上,并分别转化至酵母(INVSc1)和拟南芥(Arabidopsis thaliana)。对pYES2-Put-HVA1转化酵母进行盐碱、氧化胁迫、渗透胁迫及干旱处理,结果表明:重组酵母提高了对盐碱、氧化、渗透胁迫及干旱等逆境的适应能力。对部分转基因株系进行了抗盐性试验,表明转Put-HVA1基因拟南芥在150mmol/L NaCl和5mmol/L NaHCO3胁迫下长势优于野生型拟南芥,表现出一定的耐受性。
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| 关 键 词： | 碱茅 酵母 拟南芥 逆境 |
Cloning of a LEA Related Gene HVA1 from Alkali Grass and its Expression in Yeast and Arabidopsis |
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| Liu Xiaoli,Liu Shenkui,Tetsuo Takano.Cloning of a LEA Related Gene HVA1 from Alkali Grass and its Expression in Yeast and Arabidopsis[J].Molecular Plant Breeding,2010,8(5). |
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| Authors: | Liu Xiaoli Liu Shenkui Tetsuo Takano |
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| Affiliation: | Liu Xiaoli 1 Liu Shenkui 1 Tetsuo Takano 2 1 Alkali Soil Natural Environmental Science Center,Northeast Forestry University,Harbin,150040,2 Asian Natural Environment Science Center(ANESC),University of Tokyo,Tokyo,1880002,Japan |
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| Abstract: | We cloned the full sequence of cDNA sequence of HVA1 gene from alkali grass(Puccinellia tenuifolra) in this study.The HVA1 gene is 1 176 bp in length.It has an open reading frame(ORF) of 534 bp,encoding a protein of 177 amino acid residues,with protein molecular weight of 18.167 kD and isoelectric point of 7.80.Comparison of the deduced amino acids sequence of HVA1 porcine with those of rice and corn revealeded that the amino acidshomology 69% and 56%,respectively.At the same time,we constructed Put-HVA1 ge... |
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| Keywords: | HVA1 |
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