| 鼠抗人纤维蛋白抗体单链Fv片断的三维结构模建 |
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| 引用本文： | 王国力,刘士辉,黄君健,俞炜源,黄培堂,黄翠芬.鼠抗人纤维蛋白抗体单链Fv片断的三维结构模建[J].生物技术通讯,1996(1). |
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| 作者姓名： | 王国力 刘士辉 黄君健 俞炜源 黄培堂 黄翠芬 |
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| 作者单位： | 军事医学科学院生物工程研究所，军事医学科学院生物工程研究所，军事医学科学院生物工程研究所，军事医学科学院生物工程研究所，军事医学科学院生物工程研究所，军事医学科学院生物工程研究所 北京 100071，北京 100071，北京 100071，北京 100071，北京 100071，北京 100071 |
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| 摘 要： | 用Biosym公司开发的计算机辅助分子设计系统模建了鼠抗人纤维蛋白抗体Fv片断的三维结构。Fv是由重链可变区(Vh)和轻链可变区(Vl)两个结构域组成的具有抗原结合能力的最小抗体片断。先分别模建了Vh和Vl两个结构域,然后搭建出Fv片断的整体三维结构,并对模建的结构进行了分子力学和动力学优化。对结构的合理性验证显示模建结构是合理的。本研究为鼠抗人纤维蛋白抗体Fv片断的人源化的分子设计打下了基础。
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| 关 键 词： | 计算机辅助分子设计 同源模建 抗体Fv片断 |
Three Dimensional Structure Modelling of the Single - Chain Fv Fragment of Mouse Antibody Against Human Fibrin |
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| Wang Guoli,Liu Shihui,Huang Junjian et al.Three Dimensional Structure Modelling of the Single - Chain Fv Fragment of Mouse Antibody Against Human Fibrin[J].Letters in Biotechnology,1996(1). |
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| Authors: | Wang Guoli Liu Shihui Huang Junjian |
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| Abstract: | In the study, the three dimensional structure of the single -chain Fv fragment of mouse antibody against human fibrin, cloned by the phage display techniques in the lab, was modelled by using the computer -aided molecular design system developed by BIOSYM. Fv, the smallest antibody fragment with the competence in binding the antigen, is composed of two domains of Vh (heave variable region) and Vl(light variable region) . In the paper the Vh and Vl domains of the Fv fragment were modelled respectively by homology modelling. Based on these, the whole three -dimensional structure of the Fv was constructed. Then the model structure was refined by the methods of molecular mechanics and dynamics. The refined structure was proved reasonable by Profile-3D program. |
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| Keywords: | Computer-aided molecular design system Homology modelling Fv fragment of antibody |
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