牙科氧化锆氧化铝陶瓷复合材料的生物相容性

Biocompatibility of ZrO2 and Al2O3 ceramic composites in dentistry

BACKGROUND: The physiological environment of oral cavity is very complex. Dental restoration materials exposed to the weakly acidic body fluids in the long term, and were in the complex influences including physical, chemical, biological and mechanical and other factors. Therefore, the evaluation of biocompatibility for dental materials is the focus of research before entering the clinical trials, and also the important technical indicators to  ensure the clinical safety. OBJECTIVE: To preliminarily evaluate the biocompatibility of ZrO2 and Al2O3 ceramic composites. METHODS: The experiment was divided into six groups: ZrO2 and Al2O3 (1:1) 100% extract group, ZrO2 and Al2O3 (1:1) 50% extract group, ZrO2 and Al2O3 ( 4:1) 100% extract group, ZrO2 and Al2O3 (4:1) 50% extract group, and the volume fraction of 0.64% phenol positive control group and 100% fresh medium of negative control group. Acute hemolysis and in vitro cytotoxicity experiments were conducted according to the biological evaluation standards and requirements of GB-T16886 15-2003 medical devices. The effects of the above six groups of extracts on rabbit blood cells and L-929 mouse fibroblast cells were tested respectively. RESULTS AND CONCLUSION: There was no significant difference in the absorbance values between different concentrations of experimental groups and negative control group (P > 0.05). The cytotoxicity rating of all experimental groups was 0-1 grade. The hemolysis rates in the ZrO2 and Al2O3 (1:1) 100% extract and ZrO2 and Al2O3 (4:1) 100% extract groups were 1.27% and 2.4% respectively, less than the accepted safety standards of 5%. These results demonstrate that the acute hemolytic experiment and in vitro cytotoxicity experiment of (1:1), (4:1) ZrO2 and Al2O3 ceramic composites are all negative, and all have good cytocompatibility and blood compatibility.