晚期糖基化终末产物可影响破骨细胞的骨吸收功能

BACKGROUND: The effects of advanced glycation end products (AGEs) on osteoclast-induced bone resorption is controversial and the underlying mechanisms remain unclear. Most of the studies indicate that AGEs can enhance bone resorption, while some others show the opposite effects. OBJECTIVE:To investigate the effects of AGEs on osteoclast-induced inorganic matrix dissolution and organic component degradation and the underlying mechanisms. METHODS: RAW 264.7 cells were induced to generate osteoclasts, and AGEs (50-400 µg/mL) or control-bovine serum albumin (100 µg/mL) was added since the beginning of the induction. The effect of AGEs on bone resorption was evaluated by analyzing the area of resorption pits on the Osteo Assay Surface plates and the expression of cathepsin K. Furthermore, the number of tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cells, nuclei per osteoclasts and the expression of integrin ανβ3 were detected. RESULTS AND CONCLUSION: The area of resorption pits and expression of cathepsin K in AGEs groups were significantly decreased compared with the control group, and this inhibiting effect became more obvious with the increase of AGEs concentration. TRAP staining also showed that number of TRAP-positive multinucleated cells and nuclei per osteoclast were significantly reduced in an AGE dose-dependent manner. Quantitative PCR revealed that the expression of integrin ανβ3 decreased significantly with the extension of AGEs incubation time. These data indicate that AGEs can exert inhibitory effects on organic and inorganic matrix degradation induced by osteoclasts. The underlying mechanism may be involved in the inhibitory effects of AGEs on directed differentiation and cell fusion of osteoclast precursor cells, and migration and adhension of osteoclasts. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Advanced glycation end products influence osteoclast-induced bone resorption