乳腺癌组织中趋化因子CXCL12及其受体CXCR4、CXCR7的表达及临床意义

目的:探讨趋化因子CXCL12及其受体CXCR4、CXCR7在乳腺癌组织中的表达及临床意义。方法:应用qRT-PCR方法检测35例新鲜乳腺癌组织及癌旁正常乳腺组织中CXCL12及其受体CXCR4、CXCR7的mRNA表达,采用免疫组化检测120例乳腺癌细胞组织石蜡标本中CXCL12及其受体CXCR4、CXCR7的蛋白表达,并分析三者的表达与患者临床特征的关系。结果:qRT-PCR结果显示,CXCL12、CXCR4、CXCR7的mRNA在乳腺癌组织中表达量均明显高于癌旁正常乳腺组织(均P0.05)。免疫组化结果显示,CXCL12、CXCR4、CXCR7蛋白在乳腺癌组织中阳性表达率分别为70.8%(85/120)、65.8%(79/120)和63.3%(76/120),三者在均在伴有淋巴结转移及TNM分期较高的患者乳腺癌组织中表达明显升高(均P0.05)。结论:趋化因子CXCL12及其受体CXCR4、CXCR7的高表达可能与乳腺癌淋巴转移及恶性进展密切相关。

Expression and clinical significance of chemokine CXCL12 with its receptor CXCR4 and CXCR7 in human breast cancer tissue

Objective: To investigate the expressions of chemokine CXCL12 as well as its receptor CXCR4 and CXCR7 in breast cancer tissue and the clinical significance. Methods: The mRNA expressions of CXCL12, CXCR4 and CXCR7 were determined by qRT-PCR method in fresh specimens of breast cancer tissue and their adjacent normal breast tissue from 35 patients. The protein expressions of CXCL12, CXCR4 and CXCR7 were detected by immunohistochemical staining in 120 paraffin-embedded samples of breast cancer tissue, and the relations of their expressions with clinicopathologic features of breast cancer were analyzed. Results: Results of qRT-PCR showed that the mRNA expression levels of CXCL12, CXCR4 and CXCR7 in breast cancer tissue were all significantly higher than those in adjacent normal breast tissue (all P<0.05). Results of immunohistochemical staining showed that the positive expression rate of CXCL12, CXCR4 and CXCR7 in breast carcinoma tissues was 70.8%, 65.8% and 63.3% respectively, and the positive expression rate of either protein was significantly increased in breast cancer tissue from those with lymph node metastasis or advanced TNM stage (all P<0.05). Conclusion: The high expressions of chemokine CXCL12 as well as its receptor CXCR4 and CXCR7 may be closely associated with the lymph node metastasis and malignant progression of breast cancer.