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马铃薯新品种"云薯201"脱毒种苗生产中的影响因素研究
引用本文:杨琼芬,隋启君,李世峰,杨丽英,李先平,杨万林,白建明.马铃薯新品种"云薯201"脱毒种苗生产中的影响因素研究[J].西南农业学报,2006,19(4):679-682.
作者姓名:杨琼芬  隋启君  李世峰  杨丽英  李先平  杨万林  白建明
作者单位:云南省农业科学院马铃薯研究开发中心,云南,昆明,650205
基金项目:国家科技攻关“西部开发”重大项目(2005BA901A07)
摘    要:马铃薯脱毒种苗的生产已获得成功,但是不同的品种要求的培养条件不同。研究了影响马铃薯新品种“云薯201”进行脱毒种苗生产中的各种因素,试验结果表明,培养基以MS 赤霉素0.2 mg/L 泛酸钙0.5 mg/L NAA 0.05 mg/L为宜;接种时茎尖大小为0.1~0.2 cm时脱毒效果最好;在种苗大量扩繁时碳源浓度以20 g/L为宜,接种密度25株/瓶,选用1/2 MS的液体培养基最好。这些措施为该品种大面积推广应用提供技术依据。

关 键 词:马铃薯  脱毒苗  品种  生产
文章编号:1001-4829(2006)04-0679-04
收稿时间:2006-01-06
修稿时间:2006-01-06

Analysis of the factors affecting virus-free potato seedlings production of Yunshu 201
YANG Qiong-fen,SUI Qi-jun,LI Shi-feng,YANG Li-ying,LI Xian-ping,YANG Wan-lin,BAI Jian-ming.Analysis of the factors affecting virus-free potato seedlings production of Yunshu 201[J].Southwest China Journal of Agricultural Sciences,2006,19(4):679-682.
Authors:YANG Qiong-fen  SUI Qi-jun  LI Shi-feng  YANG Li-ying  LI Xian-ping  YANG Wan-lin  BAI Jian-ming
Affiliation:Potato Research Center, Yunnan Academy of Agricultural Sciences, Yunnan Kunming 650205, China
Abstract:It is successful in virus-free potato seedlings production,however,the culture conditions varied among different potato varieties.The factors affecting virus-free potato seedlings of new variety Yunshu 201 was analyzed.The results showed that the culture medium was better with MS including 0.2 mg/L Gibberellin 0.5 mg/L Pantothenic acid 0.05 mg/L NAA,and the impact of virus-free was perfect when inoculating with 0.1-0.2 cm stem apical point.The experiment indicated that the concentration of C and density of inoculation were 20 g/L,25 plantlets/bottle and 1/2 MS liquid medium were suitable for this variety.These results were put forward to spread the new potato variety widely.
Keywords:potato  virus-free seedlings  varieties  production
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