三种方法联合检测尿液有形成分的比较和评价

探讨Sysmex UF-50尿流式细胞仪、干化学分析仪及尿沉渣镜检三种方法检测尿液中红细胞（RBC）、白细胞（WBC）和管型（CAST）的应用及影响因素。方法对640例病人的尿液分别用UF-50尿沉渣分析仪、干化学分析仪及尿沉渣镜检三种方法进行检测,并对结果进行比较。结果 UF-50检测RBC、WBC和CAST的阳性率分别为：29.38%、24.38%和8.75%。干化学法检测RBC和WBC的阳性率是33.90%和15.1%;尿沉渣镜检法检测RBC、WBC和CAST的阳性率分别为：19.22%、20.94%和2.03%。结论 UF-50尿流式细胞仪和干化学分析仪不能取代尿沉渣镜检,建议三种方法联合应用,减少检验误差,提高尿液分析质量。

Comparison and evaluation of the combined detection of formed elements in urine with three methods

Objective To explore the applications and influencing factors in detecting urine erythrocyte,leukocyte and casts by using Urine flow cytometer Sysmex UF-50,test-strip measurement and sediment microscopy.Methods Urine specimens of 640 inpatients were collected and all the samples were examined by UF-50,test-strip and microscopy respectively,then the results of three methods were compared.Results Analyzed by UF-50,the positive rate of urine erythrocyte,leukocyte and casts were 29.38%,24.38% and 8.75% respectively;Analyzed by test-strip,the positive rate of urine erythrocyte and leukocyte were 33.90% and 15.1% respectively;Analyzed by sediment microscopy,the positive rate of urine erythrocyte,leukocyte and casts were 19.22%,20.94% and 2.03% respectively.Conclusions Sediment microscopy can not be replaced by Urine flow cytometer Sysmex UF-50 and test-strip in urine analysis.Combined application of these three methods would be optimal to improve the accuracy and precision in urine analysis.