| 灯盏花素与牛血清白蛋白相互作用的荧光光谱研究 |
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| 引用本文： | 张怀斌,马丽英,荣先国.灯盏花素与牛血清白蛋白相互作用的荧光光谱研究[J].化学研究,2012(2):13-16. |
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| 作者姓名： | 张怀斌 马丽英 荣先国 |
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| 作者单位： | 滨州医学院烟台校区药学院,山东烟台264003 |
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| 基金项目： | 滨州医学院科研专项资助项目（BY2009KJ30）. |
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| 摘 要： | 采用荧光光谱法和紫外吸收光谱法研究了灯盏花素(BR)与牛血清白蛋白(BSA)的相互作用;利用热力学方程计算了295K和308K下的热力学参数ΔH、ΔG和ΔS,根据Stern-Volmer方程求出了猝灭常数和结合常数.结果表明,BR对BSA的荧光具有猝灭作用,其猝灭机制为动态-静态联合猝灭,BSA发射峰略有蓝移.BR与BSA之间的作用力主要为疏水作用.
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| 关 键 词： | 灯盏花素 牛血清白蛋白 相互作用 荧光光谱 |
Interaction between breviscapine and bovine serum albumin studied by fluorescence spectrometry |
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| ZHANG Huai-bin,MA Li-ying,RONG Xian-guo.Interaction between breviscapine and bovine serum albumin studied by fluorescence spectrometry[J].Chemical Research,2012(2):13-16. |
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| Authors: | ZHANG Huai-bin MA Li-ying RONG Xian-guo |
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| Affiliation: | (Pharmaceutical College, Binzhou Medical University, Yaitai 264003, Shandong, China) |
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| Abstract: | The interaction between breviscapine （BR） and bovine serum albumin （BSA） was in- vestigated by means of fluorescence spectrometry and ultraviolet absorption spectrometry. The thermodynamic parameters AH, AG and AS at 295 K and 308 K were calculated using thermo- dynamic equations. The quenching constant, binding constant and binding sites were obtained according to Stern-Volmer equation. Results indicate that the fluorescence of BSA is quenched by BR, and BR functions to quench the fluorescence of BSA by a static-and-dynamic joint quenching process, with the maximum emission wavelength of BSA being slightly blue-shifted. Besides, the interaction between BR and BSA is dominated by hydrophobic force. |
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| Keywords: | breviscapine bovine serum albumin interaction fluorescence spectrometry |
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