肾癌组织中内皮细胞特异性分子ESM-1基因的原位检测和意义

目的确定ESM-1mRNA在肾癌中的定位,探讨其表达在肾癌生物学特性中的意义。方法采用地高辛标记的原位杂交技术检测ESM-1mRNA在50例肾癌、30例癌旁正常肾组织中的表达状况,并分析其与临床病理特征的关系。结果ESM-1mRNA主要定位于血管内皮细胞的胞质伴胞核中,在正常肾组织与肾癌中均有表达,部分表达在肾小管上皮和肾癌细胞。肾癌间质血管内皮的ESM-1mRNA高表达率(52.00%)显著高于正常肾组织的血管内皮(13.33%);肾透明细胞癌与颗粒细胞癌中ESM-1mRNA的表达差异有显著性,而与患者的性别、年龄、肿瘤的组织学分级、淋巴结转移和肿块大小无明显相关。结论ESM-1mRNA表达可作为新生血管内皮的标志物,可能与肾癌的血管生成及发生、发展有关。

In situ detection and significance of endothelial cell specific molecule ESM-1 gene in renal cell carcinoma

Objective To observe the histological localization of ESM-1 gene expression in renal cell carcinoma,and to discuss its clinical significance.Methods With in situ hybridization method,ESM1mRNA expression was detected and their relationships with clinic pathologic features were also analyzed in 50 cases with renal carcinoma and in 30 cases with normal renal tissues adjacent to neoplastic lesions.Results ESM-1mRNA expression was found both in renal carcinoma and in normal renal tissue, which was mainly located in the cytoplasm of vascular endothelia and partially in epithelia of renal tubule and carcinoma cells.The high expression rate of ESM-1mRNA in the endothelia of cancer mesenchymal((52.00%)) was significantly higher than that in the normal renal tissue((13.33%))(P<0.01).Significant difference was found between ESM-1mRNA high expression in granular cell carcinoma and in renal clear cell carcinoma(P<0.01),but it was not correlated to sex,age,histological grade,lymphnode involvement and tumor size.Conclusions ESM-1mRNA could be used as a potential marker of vascular endothelium,and might be involved in the development of renal carcinoma and its angiogenesis.