Aluminium speciation in cerebrospinal fluid of acutely aluminium-intoxicated dialysis patients before and after desferrioxamine treatment; a step in the understanding of the element's neurotoxicity |
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Authors: | Van Landeghem G; Haese P; Lamberts L; Barata J; DeBroe M |
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Affiliation: | Department of Nephrology-Hypertension, p/a University Hospital Antwerp, Wilrijkstraat 10, B-2650 Edegem/Antwerp, Belgium; Department of Nephrology and Internal Medicine, Hospital de Santa Cruz, Caraxide, Lisboa, Portugal; Corresponding author |
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Abstract: | Background: The association between aluminium and
dialysis encephalopathy and deterioration of the neurological state during
desferrioxamine treatment of dialysis patients is well established. At
present little is known about the speciation and the mechanisms underlying
the element's neurotoxicity. Methods. Aluminium speciation was performed in
cerebrospinal fluid samples of acutely aluminium-intoxicated dialysis
patients using a recently developed high-performance liquid
chromatographic/electro-thermal atomic absorption spectrometric hybrid
method. Results: Baseline cerebrospinal fluid
aluminium levels of samples taken shortly after the intoxication were low
but elevated (5.0±2.0 &mgr;g/l, n=3) as compared to subjects
with normal renal function (<1 &mgr;g/l). In contrast to the
situation noted in serum and to the iron speciation in cerebrospinal fluid,
aluminium was not bound to transferrin but appeared as two distinct
compounds, the main fraction eluting at the elution volume of aluminium
citrate/silicate. The second compound was not identified. Forty-four hours
after desferrioxamine administration the cerebrospinal fluid aluminium
levels had increased up to a concentration of 10.3±2.5
&mgr;g/l (n=3). This was accompanied by a change in the speciation
profile with aluminium appearing at the elution volume of aluminoxamine.
Conclusion: Our findings may contribute to a better
understanding of the neurotoxic effects of aluminium and its
desferrioxamine chelate in dialysis patients. |
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