| 茶多酚对卵巢癌SKOV3细胞自噬水平的影响及相关机制研究 |
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| 引用本文： | 詹欣,张玲云,王志华.茶多酚对卵巢癌SKOV3细胞自噬水平的影响及相关机制研究[J].浙江中西医结合杂志,2015,25(1):23-25. |
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| 作者姓名： | 詹欣 张玲云 王志华 |
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| 作者单位： | 杭州市第一人民医院妇产科 杭州 310006 |
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| 摘 要： | 目的研究茶多酚(EGCG)对人卵巢癌SKOV3细胞自噬水平的影响及相关机制。方法用EGCG处理SKOV3细胞,Western blot检测自噬相关蛋白LC3-II及蛋白激酶B(PKB)信号通路相应蛋白表达变化。结果 EGCG处理SKOV3细胞后,自噬相关蛋白LC3-II表达上调,并呈一定的时间浓度依赖性。EGCG处理SKOV3细胞后,AKT的磷酸化水平下调,AKT激活剂胰岛素样生长因子1(IGF-1)预处理后,自噬相关蛋白LC3-II高表达被抑制。结论 EGCG通过AKT介导的信号通路诱导SKOV3细胞自噬水平升高。
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| 关 键 词： | 卵巢癌 SKOV3细胞 茶多酚 PKB 自噬 |
| 收稿时间： | 2014/4/12 0:00:00 |
| 修稿时间： | 2014/11/11 0:00:00 |
The effect of EGCG-induced autophagy of person ovarian cancer cell line SKOV3 and underline mechanism |
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| ZHAN Xin,ZHANG Lingyun,WANG Zhihua.The effect of EGCG-induced autophagy of person ovarian cancer cell line SKOV3 and underline mechanism[J].Zhejiang Journal of Integrated Traditional Chinese and Western Medicine,2015,25(1):23-25. |
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| Authors: | ZHAN Xin ZHANG Lingyun WANG Zhihua |
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| Affiliation: | ZHAN Xin;ZHANG Lingyun;WANG Zhihua;Department of Obstetrics & Gynecology,Hangzhou First People’s Hospital; |
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| Abstract: | AIM: To investigate the effect of EGCG-induced autophagy of person ovarian cancer cell line SKOV3 and explore the mechanism. METHODS: After cultured person ovarian cancer cell SKOV3 were treated with EGCG, LC3-II and AKT protein expression was detected by Western blot; RESULTS: After cultured person ovarian cancer cell SKOV3 were treated with EGCG, the level of autophagy-associated protein LC3-II was up-regulated; The level of phospho-AKT was also decreased by treatment with EGCG. Pretreatment with AKT activator, insulin-like growth factor 1 (IGF-1), suppressed the EGCG-induced LC3-II expression. CONCLUSION: EGCG can induce autophagy in person ovarian cancer cell SKOV3 through AKT signaling pathway. |
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| Keywords: | ovarian cancer SKOV3 cells epigallocatechin-3-gallate PKB autophagy |
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