去铁胺对大鼠缺血心肌微血管形成的影响及可能机制

目的探讨去铁胺(DFO)对大鼠缺血心肌微血管形成的作用及可能机制。方法 48只SD大鼠随机均分为正常对照组(A组)、假手术组(B组)、结扎组(C组)和DFO 200mg/kg组(D组)。术后7d,处死大鼠,免疫组化法检测微血管密度(MVD)及低氧诱导因子1α(HIF-1α)蛋白表达,RT-PCR检测HIF-1αmRNA表达。结果与A、B组相比,C、D组MVD、HIF-1α蛋白和mRNA表达均增加(P<0.05或P<0.01),其中D组各指标增加更为显著(P<0.01)。结论 DFO可以促进大鼠缺血心肌组织微血管形成;其作用机制可能与上调HIF-1α的表达有关。

Effect and possible mechanism of desferrioxamine on microvascular formation of ischemic myocardium in rats

Objective To investigate the effect and possible mechanism of deferoxamine(DFO) on microvascular formation of ischemic myocardium in rats.Methods Forty-eight SD rats were equally randomized into four groups of A(normal controls),B(sham operated),C(ligating the left anterior descending of the coronary artery) and D(injected with DFO 200 mg/kg before the ligation).SD rats were sacrificed 7 days later.Microvessel density(MVD) and the protein expression of hypoxia-inducible factor-1α(HIF-1α) were detected by immunohistochemistry,and the mRNA expression of HIF-1α was measured by RT-PCR.Results Compared with groups of A and B,MVD and the protein and mRNA expressions of HIF-1α were all increased in groups of C and D(P<0.05 or P<0.01),the changes of which were more significant in group D(P<0.01).Conclusion Deferoxamine can promote the microvascular formation of ischemic myocardium in rats,probably via up-regulating HIF-1α expression.