DNA-fluorescence of mammalian intra-nucleolar chromatin detected by confocal laser scanning microscopy (CLSM) |
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Authors: | H Spring M F Trendelenburg M Montag |
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Affiliation: | Institute of Experimental Pathology, German Cancer Research Center, Heidelberg, Federal Republic of Germany. |
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Abstract: | The complex spatial DNA distribution in the mammalian interphase nucleus was investigated in Feulgen stained thick sections through mouse trophoblast giant nuclei after Lowicryl embedding. DNA-fluorescence was visualized using confocal laser scanning microscopy. Our results show that the spatial arrangement of major interphase chromatin areas can be precisely documented, including the distribution of small intra-nucleolar chromatin zones. |
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Keywords: | mammalian nucleolar chromatin mouse trophoblast Lowicryl embedding Feulgen fluorescence confocal laser scanning microscopy |
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