| 高效液相色谱-蒸发光散射法测定乳粉中的低聚果糖和低聚半乳糖 |
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| 引用本文： | 张 丽,张建辉,张继红,常晓途,康绍英,周兴旺. 高效液相色谱-蒸发光散射法测定乳粉中的低聚果糖和低聚半乳糖[J]. 食品安全质量检测学报, 2017, 8(12): 4729-4735 |
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| 作者姓名： | 张 丽 张建辉 张继红 常晓途 康绍英 周兴旺 |
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| 作者单位： | 湖南省食品质量监督检验研究院,湖南省食品质量监督检验研究院,湖南省食品质量监督检验研究院,长沙县食品安全检测中心,湖南省食品质量监督检验研究院,湖南省食品质量监督检验研究院 |
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| 基金项目： | 湖南省食药监局食品药品安全科技项目(编号：湘食药科R201610) |
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| 摘 要： | 目的建立一种检测乳粉中蔗果三糖、蔗果四糖、蔗果五塘、低聚半乳糖的高效液相色谱-蒸发光散射法(high performance liquid chromatography coupled with evaporative light scattering detector,HPLC-ELSD)。方法以水和乙腈为流动相梯度洗脱,利用糖柱分离,蒸发光散射检测器检测。考察了沉淀剂、酶解pH、温度和时间的影响。结果经过优化,得到以乙腈为沉淀剂,酶解pH为6.0、酶解温度为25℃、酶解时间为50min的最佳实验条件。该方法的线性范围均为0.05~2.0 mg/m L,半乳糖、乳糖、蔗果三糖、蔗果四糖、蔗果五糖检出限分别为0.02、0.02、0.01、0.02、0.02 mg/m L(S/N=3),回收率为92.52%~106.00%,相对标准偏差(relative standard deviation,RSD)低于5.12%(n=6)。结论该方法能准确可靠地检测乳粉中低聚果糖和低聚半乳糖的含量。
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| 关 键 词： | 乳粉 低聚果糖 低聚半乳糖 高效液相色谱 蒸发光散射 |
| 收稿时间： | 2017-10-12 |
| 修稿时间： | 2017-12-11 |
Determination of fructo oligosaccharide and galactooligosaccharides in milk powder by high performance liquid chromatography coupled with evaporative light scattering detector |
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| ZHANG Li,ZHANG Jian-Hui,ZHANG Ji-Hong,CHANG Xiao-Tu,KANG Shao-Ying and ZHOU Xing-Wang. Determination of fructo oligosaccharide and galactooligosaccharides in milk powder by high performance liquid chromatography coupled with evaporative light scattering detector[J]. Journal of Food Safety & Quality, 2017, 8(12): 4729-4735 |
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| Authors: | ZHANG Li ZHANG Jian-Hui ZHANG Ji-Hong CHANG Xiao-Tu KANG Shao-Ying ZHOU Xing-Wang |
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| Affiliation: | Hunan Institute of Food Quality Supervision Inspection and Research,Hunan Institute of Food Quality Supervision Inspection and Research,Hunan Institute of Food Quality Supervision Inspection and Research,Food Safety Inspection Center of Changsha County,Hunan Institute of Food Quality Supervision Inspection and Research,Hunan Institute of Food Quality Supervision Inspection and Research |
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| Abstract: | |
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| Keywords: | milk powder Fructo oligosaccharide Galactooligosaccharides high perfaormance liquid chromatography Evaporative Light-scattering Detector |
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