心肌细胞电位传感器在海洋生物毒素检测中的研究

目前海洋毒素对人类健康的威胁越加严重，尤其是麻痹性毒素。提出了一种基于细胞电位传感器检测贝类的生物毒性的新方法，采用心肌细胞与微电子加工技术的微阵列芯片（MEA）相结合，构建了新型的心肌细胞电位传感器。通过心肌细胞与MEA芯片的紧密耦合，实现了实时监测心肌细胞电位的变化，输出稳定和一致性好的胞外场电位信号。通过检测海洋中危害较大的石房蛤麻痹性毒素（STX），分析细胞的信号特征参数，实验结果表明，STX毒素对心肌细胞的电生理活动有明显的抑制作用，对脉冲幅值和脉冲频率有明显的浓度依赖性抑制作用。常规标准的生物毒性检测方法小鼠生物法的检出限为40 g/100 g，而心肌细胞电位传感器可检测的下限为1.004 ng/mL，检测范围为25 nmol/L~1600 nmol/L，通过对比表明该方法具有较好的检出限且检测方法相对简单，易于规范化和标准化。通过进一步的完善，该方法在海洋毒素的快速检测应用方面具有良好的发展前景。

Study on marine toxins detection of cardiomyocyte potential sensor

With the rapid development of modern industry,the threat from marine toxins to human health has be?come increasingly serious,especially the paralytic biological poison. This paper presents a new method of cell-based potential sensor to detect shellfish toxicity. The cardiomyocyte combined with microelectrode array(MEA)by microelectronic processing technique which construct a novel cardiomyocyte potential sensor. The sensor can not on?ly real-time monitoring,but also could output good extracellular field potential signal with stability and consistency, which cardiomyocytes seeded on the MEA high-closely. By testing saxitoxin(STX)and analyzing the characteristics of signal parameters,the experimental results illustrate the STX have obvious inhibitory effect for electrical activity of cardiomyocyte. And the peak potential amplitude and frequency have obvious inhibition of concentration depen?dence,and presented the different concentration sensitivity. The LOD(limit of detection)of conventional standard laboratory toxicity detection on mouse bioassay method is 40μg/100 g,while the LOD and detection range of cardio?myocyte potential sensor is 1.004 ng/mL and 25 nmol/L~1 600 nmol/L,respectively. By comparative experiments, this method has great LOD and the detection is relatively simple and easy to fit standardization. With further im?provement,the method has good prospects for development on detection rapidly toxicity of marine toxins.