| 丙型肝炎病毒C蛋白和NS3蛋白双表位嵌合型重组抗原的表达 |
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| 引用本文： | 王海林 金冬雁. 丙型肝炎病毒C蛋白和NS3蛋白双表位嵌合型重组抗原的表达[J]. 病毒学报, 1994, 10(4): 311-315 |
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| 作者姓名： | 王海林 金冬雁 |
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| 作者单位： | 中国预防医学科学院病毒学研究所病毒基因工程国家重点实验室 |
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| 摘 要： |
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| 关 键 词： | 丙型肝炎病毒 嵌合抗原 ELISA C蛋白 NS3蛋白 |
EXPRESSION OF CHIMERIC RECOMBINANT ANTIGENS BEARING CAPSID AND NS3 EPITOPES OF HEPATITIS C VIRUS |
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| Wang Hailin Jin Dongyan Zhou Yuan Yan Ziying Hou Yunde. EXPRESSION OF CHIMERIC RECOMBINANT ANTIGENS BEARING CAPSID AND NS3 EPITOPES OF HEPATITIS C VIRUS[J]. Chinese journal of virology, 1994, 10(4): 311-315 |
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| Authors: | Wang Hailin Jin Dongyan Zhou Yuan Yan Ziying Hou Yunde |
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| Abstract: | wo plasmids,which express chimeric recombinant antigens A(C75-C33c)and B(C33c-C75),was constructed and expressed in E. coli.The chimeric antigens were salted out and purified through ion-exchange chromatography.The immunoreactivity was tested by Western blotting,sandwich ELISA and indirect ELISA. The results indicated that the capsid and NS3 epitopes on either antigen A or B were both immunoreactive.These chimeric antigens are expected to substitute currently used mixed antigens C+C33c in HCV immunoassay. |
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| Keywords: | Hepatitis C virus(HCV) Chimeric recombinant antigen ELISA |
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