|
|||||
|
|
| RP-HPLC梯度洗脱法测定儿童Ⅱ号口服液中龙胆苦苷,盐酸巴马汀、盐酸小檗碱 | |
| 引用本文： | 申兰慧,石涛,邹爱峰,任金山,张胜强.RP-HPLC梯度洗脱法测定儿童Ⅱ号口服液中龙胆苦苷,盐酸巴马汀、盐酸小檗碱[J].中成药,2004,26(4):283-286. |
| 作者姓名： | 申兰慧 石涛 邹爱峰 任金山 张胜强 |
| 作者单位： | 1. 中国药科大学药物分析教研室,江苏,南京,210009 2. 东南大学药剂科,江苏,南京,210009 3. 南京威尔曼药物研究所,江苏,南京,210009 |
| 摘 要： | 目的:建立儿童Ⅱ号口服液(黄柏、龙胆草、知母等)中龙胆苦苷,盐酸巴马汀及盐酸小檗碱高效液相色谱测定方法.方法:以DiamonsilTMC18柱(250mm×4.6mm,5μm)为色谱柱,以乙腈-磷酸-三乙胺-水为流动相,检测波长为270nm,流速为1mL·min-1.结果:龙胆苦苷,盐酸巴马汀及盐酸小檗碱的线性范围分别为72.8～728μg·mL-1(r=0.9998),7.55～75.5μg·mL-1(r=0.9998),12.45～124.5μg·mL-1(r=0.9999),平均加样回收率(n=6)分别为98.17%,98.78%,98.60%;RSD分别为1.60%,1.35%,1.25%.结论:该方法准确,重复性好,专属性好,适合于儿童Ⅱ号的质量控制. |
| 关 键 词： | 梯度洗脱 龙胆苦苷 盐酸巴马汀 盐酸小檗碱 |
| 文章编号： | 1001-1528(2003)04-0283-03 |
Determination of gentiopicrin,palmatine hydrochloride and berberine hydrochloride in Ertong Ⅱ Oral Liquid by RP-HPLC with gradient elution |
|
| SHEN Lan hui ,SHI Tao ,ZOU Ai feng ,REN Jin shan ,ZHANG Sheng qiang.Determination of gentiopicrin,palmatine hydrochloride and berberine hydrochloride in Ertong Ⅱ Oral Liquid by RP-HPLC with gradient elution[J].Chinese Traditional Patent Medicine,2004,26(4):283-286. | |
| Authors: | SHEN Lan hui SHI Tao ZOU Ai feng REN Jin shan ZHANG Sheng qiang |
| Affiliation: | SHEN Lan hui 1,SHI Tao 2,ZOU Ai feng 3,REN Jin shan 3,ZHANG Sheng qiang 1 |
| Abstract: | |
| Keywords: | RP-HPLC |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
