细胞周期蛋白D1和P21WAF1在胃癌中的表达及其与化疗药物敏感性的关系

目的检测细胞周期蛋白（cyclin）D1、P21WAF1在胃癌组织中的表达，并探讨其与化疗药物敏感性的关系。方法采用MTT比色法观察80例胃癌原代培养细胞在体外对化疗药物羟基喜树碱（HCPT）、顺铂（DDP）、阿霉素（ADM）、氟尿嘧啶（5-Fu）及丝裂霉素（MMC）的敏感性；免疫组织化学染色检测cyclin D1和P21WAF1蛋白在胃癌组织中的表达情况。结果胃癌细胞对不同化疗药物敏感性不同：5-FU、MMC和DDP对胃癌细胞的抑制率显著高于ADM和HCPT（P〈0．05）。胃癌组织中cvclin D1和P21WAF1蛋白的阳性率分别为70．0％和47．5％。cyclin D1阳性表达者对5-Fu和HCPT的敏感性显著高于阴性表达者（P〈0．05），而P21WAF1阴性表达者对MMC、5-FU和DDP的敏感性显著高于阳性表达者（P〈0．05）。结论cyclin D1和P21WAF1蛋白与胃癌对化疗药物敏感性有关．检测其表达对于化疗药物的选择具有一定的参考价值。

Relationship of the expression of cyclin D1 and P21WAF1 with the sensitivity to chemotherapeutic drugs on gastric cancers

Objective To investigate the relationship between the sensitivity to chemotherapeutic drugs and the expression of cyclin D1 and P21WAF1 in gastric carcinoma. Methods The sensitivity of 80 samples of gastric cancer cells to HCPT, CDDP, 5-FU, ADM and MMC was evaluated with MTT assay. The protein expression of cyclin D1 and P21WAF1 was detected by immunohistochemistry. Results The sensitivity of gastric carcinoma cells to chemotherapeutic drugs was different. Inhibitory rates of tumor cells exposed to 5-FU, MMC and DDP were significantly higher than those exposed to ADM and HCPT (P<0.05). Positive expression rates of cyclin D1 and P21WAF1 in gastric cancer were 70.0% and 47.5% respectively. Cyclin D1 positive cells showed more sensitive to 5-FU and HCPT than cyclin D1 negative cells. P21WAF1 negative cells showed more sensitive to MMC, 5-FU and DDP than P21WAF1 positive cells. Conclusion Expression of cyclin D1 and P21WAF1 is related with drug-resistance of tumor. Examination of cyclinD1 and P21WAF1 would have reference value in selection of chemotherapeutic drugs.