健康志愿者口服冠心Ⅱ号煎剂后血清中药物化学成分变化研究

目的考察冠心Ⅱ号煎剂口服后化学成分在人血清中的存在状况。方法用3种预处理方法和2种高效液相色谱条件对健康人服用冠心Ⅱ号煎剂后含药血清进行指纹图谱分析,并与煎剂进行比较。结果煎剂、含药血清中的差异色谱峰数目分别不超过30个、10个,后者未检测到丹参素、原儿茶醛、川芎嗪和芍药苷。结论复方制剂中吸收入血的可测化学成分数目有限,其进入体内的化学成分可能“数目相对有限”;口服复方制剂后,原有的部分化学成分可能不以原形存在于血中。

Serum Pharmacochemistry Changes of Guan Xin Ⅱ Oral Decoction in Healthy Volunteers

OBJECTIVE:To study the presence of the chemical compositions of Guan XinⅡ(GXEH)oral decoction in human serum.METHODS:The analysis of finger prints of the medicated serum sample obtained from the volunteers treated with GXEH decoction was conducted with3different pretreatment procedures and2different HPLC methods,and which were compared with decoction.RESULTS:The numbers of the discrepant chromatographic peaks detected from the decoction and the medicated serum were respectively less than30and10,danshensu,protocatechuic aldehyde,ligustrazine,paeoniflorin were failed to be traced in the latter.CONCLUSION:The numbers of chemical compositions of the compound preparation that can be traced in serum were limited and the active components that entered into body may be relatively limited in numbers.After oral administration of compound preparation,a part of chemical compositions of which may not presented as its original form in serum.