HPLC-ELSD法同时测定连翘叶中齐墩果酸和熊果酸

目的：建立高效液相色谱-蒸发光散射检测法(high performance liquid chromatography -evaporative light scattering detector，HPLC-ELSD)法同时测定连翘叶中齐墩果酸和熊果酸。方法：色谱柱：Waters Symmetry C18(4.6mm×250mm，5μm)；柱温25℃，流动相：甲醇-0.4%冰醋酸溶液(93:7，V/V)，流速0.4mL/min；蒸发光散射检测器检测条件：漂移管温度80℃，气体压力25psi。结果：齐墩果酸在0.107～2.136μg范围内线性关系良好(r=0.9991)，熊果酸在0.179～3.584μg范围内线性关系良好(r=0.9993)；齐墩果酸和熊果酸的回收率分别为98.67%和98.56%。结论：此方法简便、准确，重现性良好，为评价连翘叶的质量提供可靠的分析方法。

Simultaneous Determination of Oleanolic Acid and Ursolic Acid in the Leaves of Forsythia suspensa by HPLC-ELSD

Objective: To establish an high performance liquid chromatography-evaporative light scattering detector(HPLCELSD) method for the simultaneous determination of oleanolic acid and ursolic acid in the leaves of Forsythia suspensa.Methods: The chromatographic separation was carried out on a Waters Symmetry C18column(4.6 mm× 250 mm,5μm) with a mobile phase composed of methanol and 0.4% acetic acid(93:7,V/V) at a flow rate of 0.4 mL/min.An evaporative light scattering detector was used to detect oleanolic acid and ursolic acid with a drift tube temperature of 80 ℃ and a gas pressure of 25 psi.Results: Both the developed calibration curves of oleanolic acid and ursolic acid revealed a good linear relationship over the ranges of 0.107-2.136 μg(r =0.9991) and 0.179-3.584μg(r= 0.9993),respectively.Therecovery ratesof oleanolicacid and ursolic acid were 98.67% and 98.56%,respectively.Conclusion: A simple,accurate and credible HPLC-ELSD method has been developed,which is suitable for the quality contro1 of the leaves of Forsythia suspensa.