日本血吸虫尾蚴成虫及虫卵的早期诊断组分抗原分析

目的寻找具有血吸虫病早期诊断价值的组分抗原.方法以感染后不同时间的兔血清,用SDS-聚丙烯酰胺凝胶电泳（SDS-PAGE）和免疫印迹试验（Western blotting）方法,对日本血吸虫尾蚴、成虫和虫卵中的可溶性抗原的成分进行免疫分析.结果可溶性尾蚴抗原（SCA）94、48、41、40 kDa和38 kDa组分抗原最早出现免疫印迹反应,能被感染后2周兔血清所识别,可被感染后3周兔血清识别的SCA 71、23 kDa组分抗原反应最强.可溶性成虫抗原（AWA）最早出现反应的组分抗原是71、58 kDa,能被感染后3周兔血清所识别.可溶性虫卵抗原（SEA）最早出现反应的组分抗原是270、151、73、69、50 kDa和24 kDa,能被感染后4周兔血清所识别.结论 SCA 94、71、48、41、40、38、23 kDa,AWA 71、58 kDa和SEA 270、151、73、69、50、24 kDa能被急性感染兔血清所识别,是具有潜在早期诊断价值的组分抗原.

Analysis of early diagnostic fraction antigens of cercariae, adult worms and eggs of Schistosoma japonicum

Objective To find out the valuable early diagnostic antigen of Schistosoma japonicum. Methods The sera of rabbits were collected at different time after the rabbits were infected with cercariae of Schitosoma japonicum. The fractions of the soluble cercaria antigen (SCA), soluble adult worm antigen (AWA) and soluble egg antigen (SEA) were separated by SDS-PAGE and recognized by Western blotting with rabbits' sera of different time of post-infection. Results In Western blotting, the bands of 94, 48, 41, 40 kDa and 38 kDa of SCA appeared the earliest and were recognized by the rabbits sera of 2-week post-infection, the bands of 71 kDa and 23 kDa of SCA reacted with the rabbits sera of 3-week post-infection strongly. The bands of 71 kDa and 58 kDa of AWA appeared the earliest and were recognized by rabbits sera of 3-week post-infection. The bands of SEA reacted earliestly to the rabbits sera of 4-week post-infection were 270, 151, 73, 69, 50 kDa and 24 kDa. Conclusion The fraction antigens of 94, 71, 48, 41, 40, 38 kDa and 23 kDa of SCA, the fraction antigens of 71 kDa and 58 kDa of AWA and the fraction antigens of 270, 151, 73, 69, 50 kDa and 24 kDa of SEA could be recognized by sera of acute infected rabbits and might have potential early immuno-diagnosis value for schistosomiasis.