人骨形态发生蛋白2，3，6，12对大鼠骨肉瘤 细胞株UMR106的作用

目的：研究人骨形态发生蛋白（human bone morphogenetic proteins, hBMP）对大鼠骨肉瘤细胞株UMR106的作用。方法：用hBMP2，3，6，12重组腺病毒（AdBMP2，3，6，12）干预大鼠骨肉瘤细胞株UMR106，利用台盼蓝拒染法、TUNEL法、吖啶橙/溴乙啶（AO/EB）荧光双染法、Transwell小室和碱性磷酸酶活性测定法分别检测细胞增殖、凋亡、迁移以及成骨分化能力的变化。结果：与空白对照组和实验对照组比较，AdBMPs的干预致骨肉瘤细胞株细胞存活率降低，并呈一定的时间依赖性；凋亡率均随时间延长而增加，并且TUNEL和 AO/EB两种检测方法的结果一致；3个检测时间点的细胞穿膜数均明显减低；碱性磷酸酶（alkaline phosphatase，ALP）活性在干预第3天开始逐渐增加，至第9天仍可观测到，以上差异均有统计学意义(P＜0.01)。结论：以腺病毒介导基因转入的作用方式，hBMP2，3，6，12可以抑制大鼠骨肉瘤细胞株UMR106的增殖和迁移,并诱导其凋亡和向成骨细胞分化。

Effect of human bone morphogenetic protein 2, 3, 6, and 12 on osteosarcoma cell line UMR106

ObjectiveTo investigate the effect of human bone morphogenetic protein (hBMPs) 2/3/6 and 12 on osteosarcoma cell UMR106.MethodsAdenovirus BMP2/3/6 and 12 (AdBMP2/3/6 and12) were used to treat the cell line. Their proliferation, apoptosis, and transmigration were detected by Trypan blue exclusion test, TdT mediated biotinylated dUTP nick end labling (TUNEL), acridine orange ethidium bromide (AO/EB) double fluorescent dye staining, and transwell room test, respectively. The alkaline phosphatase (ALP) activity was detected to reflect the differentiation of tumors. ResultsCompared with the control groups, the cell survival rate of the experimental groups treated with AdBMP2/3/6 and 12 showed a significant time dependent decrease (P＜0.01). The apoptosis indexes were increased significantly (P＜0.01) and the results from TUNEL and AO/EB method were consistent. The cell numbers of transmembrane significantly decreased at 24，48, and 72 h (P＜0.01). AdBMP2/3/6 and 12 treatment enhanced the activity of ALP activity from day 3 and this effect might still be observed up to day 9 of the treatment (P＜0.01). ConclusionhBMPs2/3/6 and 12 can inhibit the proliferation and transmigration, and induce their apoptosis and differentiation in osteosarcoma cell line UMR106.