陕北小粒黑豆皮原花青素提取工艺及抗氧化性研究

提取陕北小粒黑豆皮中原花青素,考察乙醇浓度、提取时间、提取温度和液料比对其提取得率的影响,利用Box-Behnken设计试验,建立提取过程的数学模型及二次多项式回归方程,预测出最优提取条件,并对提取的原花青素进行抗氧化性研究。结果表明,陕北小粒黑豆皮提取原花青素的优化工艺条件为提取时间4.2h、提取温度53℃、液料比22:1(mL/g)、乙醇浓度60%,在该条件下提取得率为5.380%;原花青素对DPPH自由基、羟基自由基和超氧阴离子均具有一定的清除效果,其IC50值分别为0.075,0.590,0.220mg/mL。

Study on the extraction processing and antioxidant anctivity of procyanidins from small black bean coats in Northern Shaanxi

Considering the influence of ethanol concentration, extraction time, extraction temperature and liquid-to-material ratio on the extraction rate, the experiments were proposed for procyanidins extraction, which was from small black bean coats in Northern Shaanxi, by single factor method and response surface methodology. The experiments were designed by the Box-Behnken of response surface software. The mathematical model of the extraction process and the regression equation of the model were established through experimental data. The optimum conditions of the extraction process were calculated by the mathematical model, and the antioxidant anctivity of procyanidins was also studied . The results showed that the optimum extraction conditions of the procyanidins from small black bean coats were as follows: extraction time 4.2 h, extraction temperature 53 ℃, the liquid to material ratio 22 1 (mL/g) and ethanol concentration 60%. Under the optimized condition, the average extraction rate was 5.38%. The antioxidant activity study showed that abilities of procyanidins in black bean coasts to scavenge DPPH and hydroxyl radical and superoxide anion were good, and the IC 50 were 0.075, 0.590, 0.220 mg/mL respectively.