丹凤牡丹花遗传毒理学研究

目的通过3项致突变实验,了解丹凤牡丹花的遗传毒性,并对其安全性做出评价。方法骨髓细胞微核试验:通过2次经口灌胃小鼠后观察计数含微核PCE数。Ames试验:利用试验菌株的鼠伤寒沙门氏菌,氨酸缺陷型4株进行试验,最终计数各皿回变菌落数。小鼠精子畸形试验:给予试验样品每日灌胃小鼠,连续5d,末次灌胃后30d计数畸形精子数。结果骨髓细胞微核试验、Ames试验、小鼠精子畸形试验结果均为阴性。结论丹凤牡丹花遗传毒性无毒,安全性较高。

Study on the genetic toxicology of Danfeng peony

objective To understand the genotoxicity of Danfeng peony flower by 3 mutagenic experiments and evaluate its safety. Methods Micronucleus test of bone marrow cells: The number of micronucleated PCEs was counted by two times of oral gavage mice. Ames test: The test strains of Salmonella typhimurium, four strains of acid-deficient type were used for the test, and the number of colonies of each dish was counted. Mouse sperm abnormality test: Mice were intragastrically administered daily for 5 consecutive days, and the number of abnormal sperm was counted 30 days after the last gavage. Results The results of micronucleus test of bone marrow cells, Ames test and mouse sperm abnormality test in mice were all negative. Conclusion Danfeng peony flower has no genetic toxicity, and has high safety.